Bednarczyk Martyna, Muc-Wierzgoń Małgorzata, Dzięgielewska-Gęsiak Sylwia, Waniczek Dariusz
Department of Hematology and Cancer Prevention, Medical University of Silesia in Katowice, 40-055 Katowice, Poland.
Department of Preventive Medicine, Medical University of Silesia in Katowice, 40-055 Katowice, Poland.
Biomedicines. 2023 Nov 9;11(11):3011. doi: 10.3390/biomedicines11113011.
Dysregulation of the autophagy process via ubiquitin is associated with the occurrence of a number of diseases, including cancer. The present study analyzed the changes in the transcriptional activity of autophagy-related genes and the ubiquitination process (UPS) in colorectal cancer tissue. (2) Methods: The process of measuring the transcriptional activity of autophagy-related genes was analyzed by comparing colorectal cancer samples from four clinical stages I-IV (CS I-IV) of adenocarcinoma to the control (C). The transcriptional activity of genes associated with the UPS pathway was determined via the microarray technique (HG-U133A, Affymetrix). (3) Results: Of the selected genes, only indicated statistical significance for all groups of colon cancer tissue transcriptome compared to the control. The transcriptional activity of the ) gene increased in all stages of the cancer, but the -value was only less than 0.05 in CSIV vs. C. ) and are statistically overexpressed in CSI. (4) Conclusions: The pathological expression changes in the studied proteins observed especially in the early stages of colorectal cancer suggest that the dysregulation of ubiquitination and autophagy processes occur during early neoplastic transformation. Stopping or slowing down the processes of removal of damaged proteins and their accumulation may contribute to tumor progression and poor prognosis.
通过泛素介导的自噬过程失调与包括癌症在内的多种疾病的发生有关。本研究分析了结直肠癌组织中自噬相关基因的转录活性变化以及泛素化过程（UPS）。（2）方法：通过比较腺癌四个临床分期I-IV（CS I-IV）的结直肠癌样本与对照（C），分析测量自噬相关基因转录活性的过程。通过微阵列技术（HG-U133A，Affymetrix）测定与UPS途径相关基因的转录活性。（3）结果：在所选基因中，与对照相比，仅 在所有结肠癌组织转录组组中显示出统计学意义。该）基因的转录活性在癌症的所有阶段均增加，但仅在CSIV与C相比时p值小于0.05。）和 在CSI中统计学上过度表达。（4）结论：特别是在结直肠癌早期观察到的所研究蛋白质的病理表达变化表明，泛素化和自噬过程的失调发生在肿瘤早期转化期间。停止或减缓受损蛋白质的清除过程及其积累可能有助于肿瘤进展和不良预后。
