Research Institute of Economic Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China.
Xinjiang Academy of Agricultural Sciences Kuqa County Upland Cotton Test Station, Xinjiang Academy of Agricultural Sciences, Kuqa 842000, China.
Genes (Basel). 2023 Nov 14;14(11):2077. doi: 10.3390/genes14112077.
The β-amylase () gene family encodes important enzymes that catalyze the conversion of starch to maltose in various biological processes of plants and play essential roles in regulating the growth and development of multiple plants. So far, have been extensively studied in (). However, the characteristics of the gene family in the crucial economic crop, cotton, have not been reported. In this study, 27 genes in the genome of () were identified by genome-wide identification, and they were divided into three groups according to sequence similarity and phylogenetic relationship. The gene structure, chromosome distribution, and collinearity of all genes identified in the genome of were analyzed. Further sequence alignment of the core domain of glucosyl hydrolase showed that all family genes had the glycosyl hydrolase family 14 domain. We identified the gene and preliminarily investigated its function by transcriptional sequencing analysis, qRT-PCR, and subcellular localization. These results suggested that the gene may influence fiber strength during fiber development. This systematic analysis provides new insight into the transcriptional characteristics of genes in . It may lay the foundation for further study of the function of these genes.
β-淀粉酶()基因家族编码在植物的各种生物学过程中催化淀粉转化为麦芽糖的重要酶,在调节多种植物的生长和发育中起着至关重要的作用。迄今为止,在()中已经对其进行了广泛的研究。然而,在关键的经济作物棉花中,尚未报道其基因家族的特征。在这项研究中,通过全基因组鉴定,从()基因组中鉴定出 27 个基因,并根据序列相似性和系统发育关系将它们分为三组。分析了在()基因组中鉴定的所有基因的基因结构、染色体分布和共线性。进一步对葡糖基水解酶核心结构域的序列比对表明,所有家族基因均具有糖苷水解酶家族 14 结构域。我们鉴定了基因,并通过转录组测序分析、qRT-PCR 和亚细胞定位初步研究了其功能。这些结果表明,该基因可能在纤维发育过程中影响纤维强度。这项系统分析为研究提供了在()中基因的转录特征的新视角。它可能为进一步研究这些基因的功能奠定基础。