Xu Juan, Jia Zhen, Xiao Shu, Long Cimin, Wang Leli
Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410081, China.
Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation, College of Life Sciences, Hunan Normal University, Changsha 410081, China.
Microorganisms. 2023 Oct 27;11(11):2646. doi: 10.3390/microorganisms11112646.
Pathogenic enterotoxigenic (ETEC) is a major cause of bacterial diarrhea in weaning piglets, which are vulnerable to changes in environment and feed. This study aimed to determine the effects of the ETEC challenge on piglet growth performance, diarrhea rate, jejunal microbial profile, jejunal morphology and goblet cell distribution. A total of 13 piglets from one litter were selected on postnatal day 21 and assigned to treatments with or without ETEC challenge at 1 × 10 CFUs, as ETEC group or control group, respectively. On postnatal day 28, samples were collected, followed by the detection of serum biochemical indexes and inflammatory indicators, HE staining, PAS staining and 16S rDNA gene amplicon sequencing. Results showed that the growth performance decreased, while the diarrhea rate increased for the ETEC group. The jejunum is the main segment of the injured intestine during the ETEC challenge. Compared with the control, the ETEC group displayed fewer goblet cells in the jejunum, where goblet cells are more distributed at the crypt and less distributed at the villus. In addition, ETEC piglets possessed higher abundances of the genus , genus and genus and lower abundances of the genus 2, genus and genus . In terms of alpha diversity, Chao 1 and observed features indexes were both increased for the ETEC group. Our study provides insights into jejunal histopathological impairment and microbial variation in response to ETEC infection for weaned piglets and is a valuable reference for researchers engaged in animal health research to select stress models.
致病性产肠毒素大肠杆菌（ETEC）是断奶仔猪细菌性腹泻的主要病因，断奶仔猪易受环境和饲料变化的影响。本研究旨在确定ETEC攻毒对仔猪生长性能、腹泻率、空肠微生物谱、空肠形态和杯状细胞分布的影响。在出生后第21天从一窝中挑选出13头仔猪，分别以1×10 CFUs进行有无ETEC攻毒处理，分别作为ETEC组和对照组。在出生后第28天采集样本，随后检测血清生化指标和炎症指标，进行苏木精-伊红（HE）染色、过碘酸-雪夫（PAS）染色和16S rDNA基因扩增子测序。结果表明，ETEC组的生长性能下降，而腹泻率上升。空肠是ETEC攻毒期间肠道损伤的主要部位。与对照组相比，ETEC组空肠中的杯状细胞较少，杯状细胞在隐窝处分布较多，在绒毛处分布较少。此外，感染ETEC的仔猪中，[具体菌属1]属、[具体菌属2]属和[具体菌属3]属的丰度较高，而[具体菌属4]属、[具体菌属5]属和[具体菌属6]属的丰度较低。在α多样性方面，ETEC组的Chao 1和观测特征指数均增加。我们的研究为断奶仔猪对ETEC感染的空肠组织病理学损伤和微生物变化提供了见解，对于从事动物健康研究的人员选择应激模型具有重要参考价值。
