Peng Xie, Wang Ru, Hu Liang, Zhou Qiang, Liu Yang, Yang Min, Fang Zhengfeng, Lin Yan, Xu Shengyu, Feng Bin, Li Jian, Jiang Xuemei, Zhuo Yong, Li Hua, Wu De, Che Lianqiang
1Key Laboratory for Animal Disease-Resistant Nutrition of the Ministry of Education of China, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130 People's Republic of China.
Animal Husbandry and Veterinary Department, Chengdu Agricultural College, Chengdu, Sichuan 611130 People's Republic of China.
J Anim Sci Biotechnol. 2019 Aug 21;10:72. doi: 10.1186/s40104-019-0376-z. eCollection 2019.
This study aimed to investigate the effects of oral administration of NCIMB 10415 () on intestinal development, immunological parameters and gut microbiota of neonatal piglets challenged with enterotoxigenic K88 (ETEC). A total of 96 1-day-old sow-reared piglets were randomly assigned to 2 groups, with 48 piglets in each group. The piglets were from 16 litters (6 piglets each litter), and 3 piglets each litter were allocated to the -supplemented (PRO) group, while the other 3 piglets were allocated to the control (CON) group. After colostrum intake, piglets in the PRO group were orally administrated with 3 × 10 CFU per day for a period of one week. On day 8, one piglet per litter from each group was challenged (CON+ETEC, PRO+ETEC) or not (CON-ETEC, PRO-ETEC) with ETEC in a 2 × 2 factorial arrangement of treatments. On day 10 (2 days after challenge), blood and tissue samples were obtained from piglets.
Before ETEC challenge, there were no significant differences for the average daily gain (ADG) and fecal score between the two groups of piglets. After ETEC challenge, the challenged piglets had greater fecal score compared to the non-challenged piglets, whereas administration was able to decrease the fecal score. Piglets challenged with ETEC had shorter villous height, deeper crypt depth, and reduced number of goblet cells in the jejunum and decreased mRNA abundance of in the ileum, whereas increased the percentage of lymphocytes, concentrations of IL-1β in the plasma and TNF-α in the ileal mucosa, as well as increased the mRNA abundances of innate immunity-related genes in the ileum tissue. These deleterious effects caused by ETEC were partly alleviated by feeding . In addition, piglets in PRO-ETEC group had decreased the percentage of CD8 T cells of the peripheral blood when compared to those in CON-ETEC group. Moreover, administration increased Verrucomicrobia at phylum level and decreased at genus level.
These results suggest that oral administration of alleviated the intestinal injury and diarrhea severity of neonatal piglets challenged by ETEC, partly through improving the intestinal microbiota and immune response. This offers a potential strategy of dietary intervention against intestinal impairment by ETEC in neonatal piglets.
本研究旨在调查口服NCIMB 10415（）对感染产肠毒素性K88大肠杆菌（ETEC）的新生仔猪肠道发育、免疫参数和肠道微生物群的影响。总共96头1日龄由母猪饲养的仔猪被随机分为2组，每组48头仔猪。这些仔猪来自16窝（每窝6头仔猪），每窝中的3头仔猪被分配到添加（PRO）组，而另外3头仔猪被分配到对照组（CON）。摄入初乳后，PRO组的仔猪每天口服3×10CFU，持续一周。在第8天，每组每窝的一头仔猪按照2×2析因处理设计接受（CON+ETEC，PRO+ETEC）或不接受（CON-ETEC，PRO-ETEC）ETEC攻毒。在第10天（攻毒后2天），从仔猪采集血液和组织样本。
在ETEC攻毒前，两组仔猪的平均日增重（ADG）和粪便评分没有显著差异。ETEC攻毒后，攻毒仔猪的粪便评分高于未攻毒仔猪，而给予能够降低粪便评分。感染ETEC的仔猪空肠绒毛高度较短、隐窝深度更深、杯状细胞数量减少，回肠中mRNA丰度降低，而增加了淋巴细胞百分比、血浆中IL-1β浓度和回肠黏膜中TNF-α浓度，以及回肠组织中固有免疫相关基因的mRNA丰度。ETEC引起的这些有害影响通过饲喂部分得到缓解。此外，与CON-ETEC组相比，PRO-ETEC组仔猪外周血中CD8 T细胞百分比降低。而且，给予增加了门水平的疣微菌门，降低了属水平的。
这些结果表明，口服缓解了ETEC攻毒的新生仔猪的肠道损伤和腹泻严重程度，部分是通过改善肠道微生物群和免疫反应实现的。这为新生仔猪ETEC引起的肠道损伤提供了一种潜在的饮食干预策略。
