Hu Feifei, Sun Xin, Su Yao, Huang Mingli
Department of Obstetrics, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, China.
Microorganisms. 2023 Nov 2;11(11):2686. doi: 10.3390/microorganisms11112686.
The vaginal microbiota undergoes subtle changes during pregnancy, which may affect different pregnancy responses. This study used the Illumina MiSeq high-throughput sequencing method to analyze the 16S rRNA gene amplicons of pregnant women and the vaginal microbiota structure of pregnant women at different pregnancy periods. There were a total of 15 pregnant women, with 45 samples were taken from these women, within half a year before becoming pregnant, in the last trimester, and 42 days postpartum. Before and after pregnancy, the female vaginal microbiota was mainly composed of , followed by and . The abundance of was relatively high. The α-diversity and microbial abundance were relatively low, and there was no significant difference in microbial composition between the two. After childbirth, the diversity and abundance of women's vaginal bacterial communities were higher, with a decrease in the number of and a higher abundance of , , and . There was a significant difference in the microbial community structure before and after pregnancy. This study showed that the microbiota structure of the vagina of pregnant women was similar to before pregnancy, but after childbirth, there were significant changes in the microbiota of the vagina, with a decrease in the number of probiotics and an increase in the number of harmful bacteria, increasing the risk of illness.
孕期阴道微生物群体会发生细微变化，这可能会影响不同的妊娠反应。本研究采用Illumina MiSeq高通量测序方法，分析孕妇的16S rRNA基因扩增子以及不同孕期孕妇的阴道微生物群结构。共有15名孕妇，从这些孕妇身上采集了45份样本，分别在怀孕前半年、孕晚期以及产后42天。怀孕前后，女性阴道微生物群主要由 组成，其次是 和 。 的丰度相对较高。α多样性和微生物丰度相对较低，两者之间的微生物组成无显著差异。分娩后，女性阴道细菌群落的多样性和丰度更高， 的数量减少， 、 和 的丰度更高。怀孕前后微生物群落结构存在显著差异。本研究表明，孕妇阴道的微生物群结构与怀孕前相似，但分娩后，阴道微生物群发生了显著变化，益生菌数量减少，有害细菌数量增加，患病风险增加。
