Aryee Godson, Luecke Sarah M, Dahlen Carl R, Swanson Kendall C, Amat Samat
Department of Microbiological Sciences, North Dakota State University, Fargo, ND 58108, USA.
Department of Animal Sciences, and Center for Nutrition and Pregnancy, North Dakota State University, Fargo, ND 58102, USA.
Microorganisms. 2023 Nov 10;11(11):2746. doi: 10.3390/microorganisms11112746.
Despite the extensive research conducted on ruminal methanogens and anti-methanogenic intervention strategies over the last 50 years, most of the currently researched enteric methane (CH) abatement approaches have shown limited efficacy. This is largely because of the complex nature of animal production and the ruminal environment, host genetic variability of CH production, and an incomplete understanding of the role of the ruminal microbiome in enteric CH emissions. Recent sequencing-based studies suggest the presence of methanogenic archaea in extra-gastrointestinal tract tissues, including respiratory and reproductive tracts of cattle. While these sequencing data require further verification via culture-dependent methods, the consistent identification of methanogens with relatively greater frequency in the airway and urogenital tract of cattle, as well as increasing appreciation of the microbiome-gut-organ axis together highlight the potential interactions between ruminal and extra-gastrointestinal methanogenic communities. Thus, a traditional singular focus on ruminal methanogens may not be sufficient, and a holistic approach which takes into consideration of the transfer of methanogens between ruminal, extra-gastrointestinal, and environmental microbial communities is of necessity to develop more efficient and long-term ruminal CH mitigation strategies. In the present review, we provide a holistic survey of the methanogenic archaea present in different anatomical sites of cattle and discuss potential seeding sources of the ruminal methanogens.
尽管在过去50年里对瘤胃产甲烷菌和抗产甲烷干预策略进行了广泛研究，但目前大多数研究的肠道甲烷减排方法效果有限。这主要是由于动物生产和瘤胃环境的复杂性、甲烷产生的宿主遗传变异性，以及对瘤胃微生物群在肠道甲烷排放中作用的不完全理解。最近基于测序的研究表明，在包括牛的呼吸道和生殖道在内的胃肠道外组织中存在产甲烷古菌。虽然这些测序数据需要通过依赖培养的方法进一步验证，但在牛的气道和泌尿生殖道中相对更频繁地一致鉴定出产甲烷菌，以及对微生物群-肠道-器官轴的日益重视，共同凸显了瘤胃和胃肠道外产甲烷菌群落之间的潜在相互作用。因此，传统上对瘤胃产甲烷菌的单一关注可能不够，需要一种整体方法，考虑瘤胃、胃肠道外和环境微生物群落之间产甲烷菌的转移，以制定更有效和长期的瘤胃甲烷减排策略。在本综述中，我们对牛不同解剖部位存在的产甲烷古菌进行了全面调查，并讨论了瘤胃产甲烷菌的潜在接种源。
