• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

蓬蒿蒸汽和叶酚提取物的组成及其对 t-BHP 诱导的 HepG2 细胞氧化损伤的保护机制。

Composition of Pamp. Steam and Leaf Phenolic Extract and Its Protective Mechanism on t-BHP-Induced Oxidative Damage of HepG2 Cells.

机构信息

School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.

出版信息

Molecules. 2023 Nov 8;28(22):7487. doi: 10.3390/molecules28227487.

DOI:10.3390/molecules28227487
PMID:38005209
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10672916/
Abstract

Plant phenolic compounds have attracted considerable attention because of their health benefits. This study aimed to investigate the composition and antioxidant activity of phenol extracts from Pamp. steam and leaf (PPP). The FTIR, UPLC-Q-Obtrip-MS, and HPLC-DAD methods were used to analyze the composition of PPP, and 20 phenolic compounds were preliminarily identified. Among them, the contents of hyperin, astragalin, and diosmetin levels were the highest. Treatment with PPP can significantly reduce t-BHP-induced cell damage in HepG2 cells, reactive oxygen species (ROS) accumulation, and malondialdehyde (MDA) content. Meanwhile, the superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and glutathione peroxidase (GSH-Px) activities can be increased. Moreover, PPP enhanced Nrf2 expression, which was consistent with that of heme oxygenase-1 (HO-1), glutamate-cysteine ligase catalytic subunit (GCLC), and NAD(P)H quinone oxidoreductase-1 (NQO1), whereas the expression of Keap1, the Nrf2 inhibitor, was decreased. All findings indicate that PPP can serve as a natural bioactive substance for preventing oxidative stress.

摘要

植物类多酚化合物因其对健康的益处而受到广泛关注。本研究旨在探讨来自 Pamp. steam 和叶（PPP）的酚提取物的组成和抗氧化活性。采用 FTIR、UPLC-Q-Obtrip-MS 和 HPLC-DAD 方法分析 PPP 的组成，初步鉴定出 20 种酚类化合物。其中，金丝桃苷、毛蕊异黄酮和芹菜素的含量最高。PPP 处理可显著降低 t-BHP 诱导的 HepG2 细胞损伤、活性氧（ROS）积累和丙二醛（MDA）含量。同时，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GSH-Px）的活性可以提高。此外，PPP 增强了 Nrf2 的表达，与血红素加氧酶-1（HO-1）、谷胱甘肽半胱氨酸连接酶催化亚基（GCLC）和 NAD（P）H 醌氧化还原酶-1（NQO1）的表达一致，而 Nrf2 抑制剂 Keap1 的表达降低。所有发现均表明，PPP 可作为预防氧化应激的天然生物活性物质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a972/10672916/a646257e069d/molecules-28-07487-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a972/10672916/29bcb7413439/molecules-28-07487-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a972/10672916/e145c34f9772/molecules-28-07487-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a972/10672916/9f576bc494b2/molecules-28-07487-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a972/10672916/733190d5399d/molecules-28-07487-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a972/10672916/71aa61aea26f/molecules-28-07487-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a972/10672916/a0629a47c3b3/molecules-28-07487-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a972/10672916/5449a92ad17a/molecules-28-07487-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a972/10672916/655776e84933/molecules-28-07487-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a972/10672916/a646257e069d/molecules-28-07487-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a972/10672916/29bcb7413439/molecules-28-07487-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a972/10672916/e145c34f9772/molecules-28-07487-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a972/10672916/9f576bc494b2/molecules-28-07487-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a972/10672916/733190d5399d/molecules-28-07487-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a972/10672916/71aa61aea26f/molecules-28-07487-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a972/10672916/a0629a47c3b3/molecules-28-07487-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a972/10672916/5449a92ad17a/molecules-28-07487-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a972/10672916/655776e84933/molecules-28-07487-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a972/10672916/a646257e069d/molecules-28-07487-g009.jpg

相似文献

1
Composition of Pamp. Steam and Leaf Phenolic Extract and Its Protective Mechanism on t-BHP-Induced Oxidative Damage of HepG2 Cells.蓬蒿蒸汽和叶酚提取物的组成及其对 t-BHP 诱导的 HepG2 细胞氧化损伤的保护机制。
Molecules. 2023 Nov 8;28(22):7487. doi: 10.3390/molecules28227487.
2
Daphnetin-mediated Nrf2 antioxidant signaling pathways ameliorate tert-butyl hydroperoxide (t-BHP)-induced mitochondrial dysfunction and cell death.瑞香素介导的Nrf2抗氧化信号通路可改善叔丁基过氧化氢（t-BHP）诱导的线粒体功能障碍和细胞死亡。
Free Radic Biol Med. 2017 May;106:38-52. doi: 10.1016/j.freeradbiomed.2017.02.016. Epub 2017 Feb 7.
3
Gallic acid, a natural polyphenol, protects against tert-butyl hydroperoxide- induced hepatotoxicity by activating ERK-Nrf2-Keap1-mediated antioxidative response.没食子酸是一种天然多酚，通过激活 ERK-Nrf2-Keap1 介导的抗氧化反应来防止叔丁基过氧化物诱导的肝毒性。
Food Chem Toxicol. 2018 Sep;119:479-488. doi: 10.1016/j.fct.2017.10.033. Epub 2017 Oct 21.
4
Isoflavone biochanin A, a novel nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant response element activator, protects against oxidative damage in HepG2 cells.染料木黄酮生物胆素 A，一种新型核因子红细胞 2 相关因子 2（Nrf2）-抗氧化反应元件激活剂，可防止 HepG2 细胞的氧化损伤。
Biofactors. 2019 Jul;45(4):563-574. doi: 10.1002/biof.1514. Epub 2019 May 27.
5
Caffeic acid prevents acetaminophen-induced liver injury by activating the Keap1-Nrf2 antioxidative defense system.咖啡酸通过激活Keap1-Nrf2抗氧化防御系统来预防对乙酰氨基酚诱导的肝损伤。
Free Radic Biol Med. 2016 Feb;91:236-46. doi: 10.1016/j.freeradbiomed.2015.12.024. Epub 2015 Dec 23.
6
[Protective effects of polysaccharides on alcohol-induced injury of HepG2 cells and its mechanisms].多糖对酒精诱导的HepG2细胞损伤的保护作用及其机制
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2022 Sep;38(3):227-232. doi: 10.12047/j.cjap.6287.2022.047.
7
Asiatic acid enhances Nrf2 signaling to protect HepG2 cells from oxidative damage through Akt and ERK activation.齐墩果酸通过激活Akt和ERK增强Nrf2信号通路，保护HepG2细胞免受氧化损伤。
Biomed Pharmacother. 2017 Apr;88:252-259. doi: 10.1016/j.biopha.2017.01.067. Epub 2017 Jan 19.
8
Antioxidant Activity of Novel Casein-Derived Peptides with Microbial Proteases as Characterized via Keap1-Nrf2 Pathway in HepG2 Cells.新型酪蛋白衍生肽的抗氧化活性及其通过 Keap1-Nrf2 通路在 HepG2 细胞中的作用。
J Microbiol Biotechnol. 2021 Aug 28;31(8):1163-1174. doi: 10.4014/jmb.2104.04013.
9
[Mechanism of Tibetan medicine Ershiwuwei Songshi Pills against liver injury induced by acetaminophen in mice based on Keap1/Nrf2 and TLR4/NF-κB p65 signaling pathways].基于Keap1/Nrf2和TLR4/NF-κB p65信号通路探讨藏药二十五味松石丸对小鼠对乙酰氨基酚所致肝损伤的作用机制
Zhongguo Zhong Yao Za Zhi. 2022 Apr;47(8):2049-2055. doi: 10.19540/j.cnki.cjcmm.20211103.707.
10
l-Methionine activates Nrf2-ARE pathway to induce endogenous antioxidant activity for depressing ROS-derived oxidative stress in growing rats.l-蛋氨酸通过激活 Nrf2-ARE 通路诱导内源性抗氧化活性，从而抑制生长大鼠中 ROS 衍生的氧化应激。
J Sci Food Agric. 2019 Aug 15;99(10):4849-4862. doi: 10.1002/jsfa.9757. Epub 2019 May 13.

引用本文的文献

1
Hepatoprotective effects of Acanthopanax trifoliatus standardized leaf extract and its formulations in a HepG2 model.刺三加标准化叶提取物及其制剂在HepG2模型中的肝脏保护作用。
BMC Complement Med Ther. 2025 Sep 1;25(1):319. doi: 10.1186/s12906-025-05025-3.

本文引用的文献

1
Antioxidant Properties of Selected Flavonoids in Binary Mixtures-Considerations on Myricetin, Kaempferol and Quercetin.某些黄酮类化合物二元混合物的抗氧化性能——杨梅素、山奈酚和槲皮素的考虑因素。
Int J Mol Sci. 2023 Jun 13;24(12):10070. doi: 10.3390/ijms241210070.
2
Comparative analysis of flavonoids, polyphenols and volatiles in roots, stems and leaves of five mangroves.五种红树科植物根、茎、叶中类黄酮、多酚和挥发物的比较分析。
PeerJ. 2023 Jun 22;11:e15529. doi: 10.7717/peerj.15529. eCollection 2023.
3
Chlorogenic Acid Attenuates Doxorubicin-Induced Oxidative Stress and Markers of Apoptosis in Cardiomyocytes via Nrf2/HO-1 and Dityrosine Signaling.
绿原酸通过Nrf2/HO-1和二酪氨酸信号通路减轻阿霉素诱导的心肌细胞氧化应激和凋亡标志物。
J Pers Med. 2023 Apr 10;13(4):649. doi: 10.3390/jpm13040649.
4
Sustainable extraction of ligustilide and ferulic acid from Angelicae Sinensis Radix, for antioxidant and anti-inflammatory activities.从当归中可持续提取藁本内酯和阿魏酸，用于抗氧化和抗炎活性。
Ultrason Sonochem. 2023 Mar;94:106344. doi: 10.1016/j.ultsonch.2023.106344. Epub 2023 Feb 24.
5
Evaluation of var. Santi (Caprifoliaceae) Stem and Leaf in Terms of Anatomical Structures and Some Phenolic Compounds.接骨木属（忍冬科）茎和叶的解剖结构及部分酚类化合物评价
Turk J Pharm Sci. 2022 Dec 21;19(6):636-641. doi: 10.4274/tjps.galenos.2021.71636.
6
Antioxidant Activity of Areca Nut Polyphenol Extracts on RAW264.7 Cells.槟榔多酚提取物对RAW264.7细胞的抗氧化活性
Foods. 2022 Nov 12;11(22):3607. doi: 10.3390/foods11223607.
7
The Antioxidant and Hypolipidemic Effects of Extracts.提取物的抗氧化和降血脂作用。
Molecules. 2022 May 26;27(11):3423. doi: 10.3390/molecules27113423.
8
Phenolic Compounds From the Stems and Leaves of (L.) DC.来自（L.）DC. 茎和叶的酚类化合物
Front Chem. 2022 Apr 14;10:889441. doi: 10.3389/fchem.2022.889441. eCollection 2022.
9
A comprehensive study of three species of Paeonia stem and leaf phytochemicals, and their antioxidant activities.全面研究三种牡丹茎叶的植物化学物质及其抗氧化活性。
J Ethnopharmacol. 2021 Jun 12;273:113985. doi: 10.1016/j.jep.2021.113985. Epub 2021 Mar 2.
10
Crm1-Dependent Nuclear Export of Bach1 is Involved in the Protective Effect of Hyperoside on Oxidative Damage in Hepatocytes and CCl-induced Acute Liver Injury.Bach1依赖Crm1的核输出参与了金丝桃苷对肝细胞氧化损伤和四氯化碳诱导的急性肝损伤的保护作用。
J Inflamm Res. 2021 Feb 25;14:551-565. doi: 10.2147/JIR.S279249. eCollection 2021.