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月桂科植物精油成分和抗氧化能力的季节性变化:1-硝基-2-苯乙烷的可靠来源

Seasonal Variation in Essential Oil Composition and Antioxidant Capacity of (Lauraceae): A Reliable Source of 1-Nitro-2-phenylethane.

作者信息

Cruz Ellen de Nazaré S da, Barros Luana de Sousa P, Guimarães Bruna de A, Mourão Rosa Helena V, Maia José Guilherme S, Setzer William N, da Silva Joyce Kelly do R, Figueiredo Pablo Luis B

机构信息

Programa Institucional de Bolsas de Iniciação Científica, Universidade Federal do Pará, Belem 66075-900, Brazil.

Laboratório de Química dos Produtos Naturais, Centro de Ciências Biológicas e da Saúde, Universidade do Estado do Pará, Belem 66087-662, Brazil.

出版信息

Molecules. 2023 Nov 14;28(22):7573. doi: 10.3390/molecules28227573.

Abstract

(Kunth) Mez essential oil has many biological activities due to its main compound 1-nitro-2-phenylethane (1N2F), followed by methyleugenol, a carcinogenic agent. This study analyzed the influence of seasonality on yields, antioxidant capacity, and 1N2F content of leaf and twig essential oils. Essential oils (EOs) were extracted with hydrodistillation and analyzed with gas chromatography coupled to mass spectrometry and a flame ionization detector. Antioxidant capacity was measured using the free radical scavenging method (DPPH). Chemometric analyses were carried out to verify the influence of climatic factors on the production and composition of EOs. 1-Nitro-2-phenylethane was the major constituent in EOs throughout the seasonal period (68.0-89.9%); methyleugenol was not detected. Essential oil yields and the 1N2F average did not show a statistically significant difference between the dry and rainy seasons in leaves and twigs. Moderate and significant correlations between major compounds and climate factor were observed. The twig oils (36.0 ± 5.9%) a showed greater antioxidant capacity than the leaf oils (20.4 ± 5.0%). The PCA and HCA analyses showed no statistical differences between the oil samples from the dry and rainy seasons. The absence of methyleugenolin in all months of study, described for the first time, makes this specimen a reliable source of 1N2F.

摘要

昆特肉豆蔻精油具有多种生物活性,这归因于其主要成分1-硝基-2-苯乙烷(1N2F),其次是致癌剂甲基丁香酚。本研究分析了季节性对叶片和嫩枝精油产量、抗氧化能力以及1N2F含量的影响。采用水蒸馏法提取精油,并用气相色谱-质谱联用仪和火焰离子化检测器进行分析。采用自由基清除法(DPPH)测定抗氧化能力。进行化学计量分析以验证气候因素对精油产量和成分的影响。在整个季节期间,1-硝基-2-苯乙烷是精油中的主要成分(68.0-89.9%);未检测到甲基丁香酚。叶片和嫩枝中精油产量和1N2F平均值在旱季和雨季之间没有统计学上的显著差异。观察到主要化合物与气候因素之间存在中度和显著的相关性。嫩枝精油(36.0±5.9%)比叶片精油(20.4±5.0%)表现出更强的抗氧化能力。主成分分析(PCA)和聚类分析(HCA)表明,旱季和雨季的油样之间没有统计学差异。本研究首次描述了在所有研究月份中均未检测到甲基丁香酚,这使得该样本成为1N2F的可靠来源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3806/10674907/f8b17676d104/molecules-28-07573-g001.jpg

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