Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal.
Laboratório Associado para a Sustentabilidade e Tecnologia em Regiões de Montanha (SusTEC), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, Portugal.
Molecules. 2023 Nov 15;28(22):7601. doi: 10.3390/molecules28227601.
Nowadays, bee products are commended by consumers for their medicinal and dietary properties. This study aimed to differentiate between monofloral bee pollens originating from Portugal using phenolic and volatile profiles and investigate their antioxidant and cytotoxic activity. Total phenolic and flavonoid compounds were recorded between 2.9-35.8 mg GAE/g and 0.7-4.8 mg QE/g, respectively. The LC/DAD/ESI-MS analytical results allowed us to identify and quantify a total of 72 compounds, including phenolic and phenylamide compounds, whereas GC-MS results revealed the presence of 49 different compounds, mostly ketones, aldehydes, esters, hydrocarbons, and terpenes. The highest DPPH radical scavenging activity, EC: 0.07 mg/mL, was recorded in the sample dominated by sp. pollen, whereas the sp. (1.59 mM Trolox/mg) and sp. (0.09 mg GAE/g) pollen species exhibited the highest antioxidant activity in ABTS and reducing power assays, respectively. Regarding the anti-carcinogenic activity, only sp. showed remarkable cytotoxic potential against MCF-7.
如今，蜂产品因其药用和饮食特性而受到消费者的称赞。本研究旨在利用酚类和挥发性成分的特征来区分原产于葡萄牙的单花种花粉，并研究其抗氧化和细胞毒性活性。总酚和类黄酮化合物的含量分别为 2.9-35.8mg GAE/g 和 0.7-4.8mg QE/g。LC/DAD/ESI-MS 分析结果共鉴定和定量了 72 种化合物，包括酚类和苯甲酰胺类化合物，而 GC-MS 结果显示存在 49 种不同的化合物，主要为酮类、醛类、酯类、烃类和萜类化合物。在以 sp.花粉为主的样品中，DPPH 自由基清除活性最高，EC：0.07mg/mL，而 sp.（1.59mM Trolox/mg）和 sp.（0.09mg GAE/g）花粉在 ABTS 和还原力测定中显示出最高的抗氧化活性。关于抗癌活性，只有 sp.对 MCF-7 表现出显著的细胞毒性潜力。
