Chekuri Sudhakar, Vyshnava Satyanarayana Swamy, Somisetti Swarupa Lakshmi, Cheniya Sai Bindu Karamthote, Gandu Chakradhar, Anupalli Roja Rani
Department of Genetics and Biotechnology, University College of Science, Osmania University, Hyderabad, Telangana 500007 India.
Department of Biotechnology, University College of Science, Sri Krishnadevaraya University, Anantapuramu, Andhra Pradesh 515003 India.
3 Biotech. 2023 Aug;13(8):289. doi: 10.1007/s13205-023-03705-w. Epub 2023 Aug 2.
In this study, the active components of the plant were carefully extracted and identified using three solvent systems. After extraction, we used solvent systems to further purify the main flavonoid chemical constituent. As a result of our analytical strategy, which included HPLC analysis, MS/MS spectroscopic analysis, and NMR data-based constructions, quercetin was determined to be the main chemical constituent. Our study suggests the potential therapeutic advantages of quercetin, a compound found in the leaves of , for treating breast cancer cell lines MCF-7 and MDA-MB-231. Our comparison of Quercetin to the regularly prescribed medicine Doxorubicin shows that it has the capacity to inhibit MCF-7 and MDA-MB-231 cells. Measurements of apoptosis and cell cycle phase showed this to be the case. Furthermore, a ladder that formed as a result of cellular damage brought on by ROS provided further proof of the drug's impact on DNA integrity. Notably, pro-apoptotic proteins displayed increased apoptosis activity in cells treated with quercetin. Given that it is extracted from plants and has less adverse effects than other compounds, quercetin is a viable option for further clinical study. The objective is to fight breast cancer, one of the most prevalent diseases in the world and a main cause of death for women. Thus, our research makes a significant addition to the ongoing search for potent, plant-based breast cancer treatments.
The online version contains supplementary material available at 10.1007/s13205-023-03705-w.
在本研究中，使用三种溶剂系统仔细提取并鉴定了该植物的活性成分。提取后，我们使用溶剂系统进一步纯化主要的黄酮类化学成分。通过包括高效液相色谱分析、串联质谱光谱分析以及基于核磁共振数据构建的分析策略，确定槲皮素为主要化学成分。我们的研究表明，槲皮素这种存在于[植物名称]叶子中的化合物，在治疗乳腺癌细胞系MCF - 7和MDA - MB - 231方面具有潜在的治疗优势。我们将槲皮素与常规处方药阿霉素进行比较，发现它有抑制MCF - 7和MDA - MB - 231细胞的能力。细胞凋亡和细胞周期阶段的测量结果证实了这一点。此外，由活性氧引起的细胞损伤导致的DNA梯形条带进一步证明了该药物对DNA完整性的影响。值得注意的是，促凋亡蛋白在用槲皮素处理的细胞中显示出增加的凋亡活性。鉴于槲皮素是从植物中提取的，且副作用比其他化合物少，它是进一步临床研究的一个可行选择。目标是对抗乳腺癌，这是世界上最普遍的疾病之一，也是女性死亡的主要原因。因此，我们的研究为正在进行的寻找有效的、基于植物的乳腺癌治疗方法的工作做出了重要贡献。
在线版本包含可在10.1007/s13205 - 023 - 03705 - w获取的补充材料。
