Jaradat Nidal, Al-Maharik Nawaf, Hawash Mohammed, Qadi Mohammad, Issa Linda, Anaya Rashad, Daraghmeh Ayham, Hijleh Lobna, Daraghmeh Tasneem, Alyat Amal, Aboturabi Ro'a
Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, Nablus P.O. Box 7, Palestine.
Department of Chemistry, Faculty of Sciences, An-Najah National University, Nablus P.O. Box 7, Palestine.
Plants (Basel). 2023 Nov 8;12(22):3805. doi: 10.3390/plants12223805.
Dehnh is a tree species that is commonly used for various purposes, including forestry, agroforestry, and conservation. The present investigation was designed to determine the composition of leaves essential oil and estimate its free radicals, porcine pancreatic lipase, α-amylase inhibitory, and antimicrobial properties in vitro. The chemical constituents were analyzed using the gas chromatography-mass spectrometry (GC-MS) technique. DPPH (2,2-diphenyl-1-picrylhydrazyl), p-nitrophenyl butyrate, and 3,5-dinitro salicylic acid (DNSA) methods were employed to estimate the antioxidant, antiobesity, and antidiabetic effects of the essential oil. The microdilution assay was employed to assess the antimicrobial efficacy of the substance against a total of seven distinct microbial species. The GC-MS results revealed that essential oil contains 52 components that makeup 100% of the entire oil. The main chemical constituents in essential oil are -cymene (38.64%), followed by aromadendrene (29.65%), and 1,8-cineol (6.45%), with monocyclic monoterpene being the most abundant phytochemical group, followed by the sesquiterpene hydrocarbon group, representing 44.27 and 31.46%, respectively. The essential oil showed a weak antioxidant effect and had no antilipase or antiamylase effects. At the same time, the oil showed a strong antimicrobial effect against methicillin-resistant (MRSA), , and , which was even more potent than the positive controls, ciprofloxacin and ampicillin, which had MIC doses of 0.2 ± 0.01, 0.2 ± 0.01, and 6.25 ± 0.1 µg/mL, respectively. It also has a strong anti- effect with a MIC of 0.2 ± 0.01 µg/mL. In light of these findings, in vivo studies should be conducted to determine the efficiency of the essential oil in treating microbial infections.
德恩树是一种常用于多种用途的树种，包括林业、农林复合经营和保护。本研究旨在确定树叶精油的成分，并在体外评估其自由基、猪胰脂肪酶、α -淀粉酶抑制和抗菌特性。使用气相色谱 - 质谱联用（GC - MS）技术分析化学成分。采用DPPH（2,2 - 二苯基 - 1 - 苦基肼）、对硝基苯基丁酸酯和3,5 - 二硝基水杨酸（DNSA）方法来评估精油的抗氧化、抗肥胖和抗糖尿病作用。采用微量稀释法评估该物质对总共七种不同微生物物种的抗菌效果。GC - MS结果显示，精油含有52种成分，占整个油的100%。精油中的主要化学成分是对异丙基甲苯（38.64%），其次是香树烯（29.65%）和1,8 - 桉叶素（6.45%），单环单萜是最丰富的植物化学基团，其次是倍半萜烃基团，分别占44.27%和31.46%。该精油显示出较弱的抗氧化作用，没有抗脂肪酶或抗淀粉酶作用。同时，该油对耐甲氧西林金黄色葡萄球菌（MRSA）等显示出很强的抗菌作用，甚至比阳性对照环丙沙星和氨苄西林更强，它们的最低抑菌浓度（MIC）剂量分别为0.2±0.01、0.2±0.01和6.25±0.1μg/mL。它对[此处原文缺失具体微生物名称]也有很强的抗菌作用，MIC为0.2±0.01μg/mL。鉴于这些发现，应进行体内研究以确定德恩树精油治疗微生物感染的有效性。
