水稻抗衰老物理和化学标记的基因位点挖掘及候选基因分析

The Mining of Genetic Loci and the Analysis of Candidate Genes to Identify the Physical and Chemical Markers of Anti-Senescence in Rice.

作者信息

Yin Wenjing, Huang Zhao, Zhong Qianqian, Tang Luyao, Wu Richeng, Li Sanfeng, Mao Yijian, Zhu Xudong, Wang Changchun, Rao Yuchun, Wang Yuexing

机构信息

National Key Laboratory of Rice Biological Breeding, China National Rice Research Institute, Hangzhou 310006, China.

College of Life Sciences, Zhejiang Normal University, Jinhua 321004, China.

出版信息

Plants (Basel). 2023 Nov 9;12(22):3812. doi: 10.3390/plants12223812.

DOI:10.3390/plants12223812

PMID:38005709

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10674301/

Abstract

Premature senescence is a common occurrence in rice production, and seriously affects rice plants' nutrient utilization and growth. A total of 120 recombinant inbred lines (RILs) were obtained from successive self-crossing of F generations derived from Huazhan and Nekken2. The superoxide dismutase (SOD) activity, malondialdehyde (MDA), content and catalase (CAT) activity related to the anti-senescence traits and enzyme activity index of rice were measured for QTL mapping using 4858 SNPs. Thirteen QTLs related to anti-senescence were found, among which the highest LOD score was 5.70. Eighteen anti-senescence-related genes were found in these regions, and ten of them differed significantly between the parents. It was inferred that , , and became involved in the regulation of the anti-senescence molecular network upon upregulation of their expression levels. The identified anti-senescence-related QTLs and candidate genes provide a genetic basis for further research on the mechanism of the molecular network that regulates premature senescence.

摘要

早衰是水稻生产中常见的现象，严重影响水稻植株的养分利用和生长。通过华占和根肯2杂交F代连续自交获得了120个重组自交系（RIL）。利用4858个单核苷酸多态性（SNP）对与水稻抗衰老性状相关的超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量和过氧化氢酶（CAT）活性以及酶活性指标进行测定，进行QTL定位。共发现13个与抗衰老相关的QTL，其中最高LOD值为5.70。在这些区域发现了18个与抗衰老相关的基因，其中10个在亲本间差异显著。推测、和在表达水平上调后参与了抗衰老分子网络的调控。所鉴定的抗衰老相关QTL和候选基因为进一步研究调控早衰分子网络的机制提供了遗传基础。