Shanghai Key Laboratory of Forensic Medicine, Key Laboratory of Forensic Science, Ministry of Justice, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China.
School of Forensic Medicine, Xi'an Jiaotong University, Xi'an 710061, China.
Fa Yi Xue Za Zhi. 2023 Oct 25;39(5):452-456. doi: 10.12116/j.issn.1004-5619.2022.320306.
To establish a simple and rapid qualitative and quantitative detection method of dexmedetomidine in blood.
Blood was separated on the Allure PFP Propyl liquid chromatography column with isocratic elution after it was precipitated by acetonitrile and filtered. Qualitative and quantitative analysis of dexmedetomidine was performed using positive ion scan mode and multi-reaction monitoring mode.
The limit of detection of dexmedetomidine in blood was 0.2 ng/mL and the limit of quantification was 0.5 ng/mL. The linearity of the method was good in the range of 0.5-1 000 ng/mL, and the correlation coefficient was greater than 0.99. The accuracy of the method was 90.34%-112.67% and the extraction recovery was 50.05%-91.08%, with no significant matrix effect.
This method is simple, selective and suitable for the qualitative and quantitative analysis of dexmedetomidine in blood, which can provide a reference for drug-facilitated cases involving dexmedetomidine.
建立一种简单、快速检测血液中右美托咪定的定性和定量方法。
血液经乙腈沉淀、过滤后，采用 Allure PFP Propyl 液相色谱柱等度洗脱，采用正离子扫描模式和多反应监测模式进行右美托咪定的定性和定量分析。
血液中右美托咪定的检测限为 0.2ng/mL，定量限为 0.5ng/mL。方法在 0.51000ng/mL 范围内线性良好，相关系数大于 0.99。方法的准确度为 90.34%112.67%，提取回收率为 50.05%~91.08%，无明显基质效应。
该方法简单、选择性好，适用于血液中右美托咪定的定性和定量分析，可为涉及右美托咪定的药物辅助案例提供参考。
