Jurkevicz Carolina Stiegler, Porto Flavia Vitorino de Araujo, Tischer Cesar Augusto, Fronza Marcio, Endringer Denise Coutinho, Ribeiro-Viana Renato Márcio
Programa de Pós-graduação em Ciência e Engenharia de Materiais (PPGCEM), Universidade Tecnológica Federal do Paraná, UTFPR-Ld, CEP 86036-370, Londrina, PR, Brazil.
Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Vila Velha - UVV, CEP 29102-920, Vila Velha, ES, Brazil.
J Pharm Sci. 2024 Feb;113(2):427-433. doi: 10.1016/j.xphs.2023.11.015. Epub 2023 Nov 24.
Ideally, the dressings used in the clinic have characteristics that help the wound closure process. Among several factors that affect the success of this healing process, there is debridement. It manages the wound bed components and the re-epithelialization process. Still, the property of debridement is not generally associated with dressings. Here, we show a chemically modified bacterial cellulose film conjugated to a proteolytic enzyme, papain, as a dressing with debridement properties. Bacterial cellulose films were reacted with a spacer derived from succinic acid and finally had this enzyme covalently immobilized in its structure by an amide bond. FT-IR and UV-vis showed bands typically of bioconjugated polymer. Enzymatic immobilization was very effective under the conditions applied with high yield (33% w/w), and these remained activated after the coupling reaction. The bacterial cellulose film with the enzyme papain attached to it was also very compatible with fibroblast cells, suggesting that it could be a promising wound dressing material for future research.
理想情况下，临床中使用的敷料应具有有助于伤口愈合过程的特性。在影响这一愈合过程成功的几个因素中，清创术是其中之一。它控制着伤口床成分和再上皮化过程。然而，清创特性通常与敷料并无关联。在此，我们展示了一种化学修饰的细菌纤维素膜，它与一种蛋白水解酶木瓜蛋白酶结合，作为一种具有清创特性的敷料。细菌纤维素膜与一种源自琥珀酸的间隔基发生反应，最终通过酰胺键将这种酶共价固定在其结构中。傅里叶变换红外光谱（FT-IR）和紫外可见光谱（UV-vis）显示出生物共轭聚合物的典型谱带。在应用的条件下，酶的固定化非常有效，产率很高（33% w/w），并且在偶联反应后这些酶仍保持活性。附着有木瓜蛋白酶的细菌纤维素膜与成纤维细胞也非常相容，这表明它可能是未来研究中一种有前景的伤口敷料材料。
