Department of Clinical Biochemistry, Department of Basic Medical Sciences, College of Medicine, Shaqra University, Dawadmi, Saudi Arabia.
Molecular Genetics and Enzymology Department, Human Genetics and Genome Research Institute, National Research Center, Dokki, Cairo, Egypt.
Skin Res Technol. 2023 Nov;29(11):e13503. doi: 10.1111/srt.13503.
Insulin resistance (IR) is a condition where cells become resistant to insulin, causing impaired glucose uptake and increased blood glucose levels. Interleukin-12 (IL-12), a cytokine, regulates the immune system. High levels of IL-12 can lead to chronic inflammation, exacerbate resistance to insulin, and contribute to type 2 diabetes. Also, link IR to acne vulgaris (AV), as it reduces tissue sensitivity to insulin, causing increased insulin levels and sebum production, which can contribute to acne development.
To explore the role of IL-12 gene expression on IR in AV patients and to study the role of IL-12 gene in the development of AV.
A case-control study was performed on 68 AV patients and 68 healthy controls. The biochemical analysis included fasting glucose, fasting insulin, (HOMA-IR), and serum IL-12 level. IL-12 gene expression was performed by quantitative real-time PCR for both two groups. In addition, folding change was calculated by using the standard 2-(∆∆Ct) method.
IL-12 level, IL-12 folding change, fasting insulin, and IR were all increased in acne patients. A highly significant linear correlation was found between IL-12 folding change and both IL-12 levels and IR. There is a substantial positive significant simple linear association between IL-12 level and IL-12 folding change, as well as IR and IL-12 folding change, in moderate and severe acne.
IL-12 gene has an important role in IR and the development of acne in AV patients.
胰岛素抵抗（IR）是一种细胞对胰岛素产生抗性的情况，导致葡萄糖摄取受损和血糖水平升高。白细胞介素-12（IL-12）是一种细胞因子，调节免疫系统。高水平的 IL-12 可导致慢性炎症，加剧对胰岛素的抵抗，并导致 2 型糖尿病。此外，将 IR 与寻常痤疮（AV）联系起来，因为它降低了组织对胰岛素的敏感性，导致胰岛素水平和皮脂分泌增加，这可能导致痤疮的发展。
探讨白细胞介素-12 基因表达对 AV 患者 IR 的作用，并研究白细胞介素-12 基因在 AV 发病机制中的作用。
对 68 例 AV 患者和 68 例健康对照进行病例对照研究。生化分析包括空腹血糖、空腹胰岛素、（HOMA-IR）和血清 IL-12 水平。对两组进行白细胞介素-12 基因表达的实时定量 PCR。此外，使用标准 2-（∆∆Ct）法计算折叠变化。
痤疮患者的 IL-12 水平、IL-12 折叠变化、空腹胰岛素和 IR 均升高。IL-12 折叠变化与 IL-12 水平和 IR 之间存在高度显著的线性相关性。中重度痤疮患者的 IL-12 水平和 IL-12 折叠变化之间以及 IR 和 IL-12 折叠变化之间存在显著的正简单线性关联。
白细胞介素-12 基因在 AV 患者的 IR 和痤疮发病机制中具有重要作用。
