Mohammed Suhayla K, Taha Ekhlass M, Muhi Samer Abdulhasan
Department of Chemistry, College of Science for Women, University of Baghdad, Al-Jadriya, Baghdad, 10001 Iraq.
Al-Yarmouk Teaching Hospital- Ministry of Health of Iraq, Baghdad, Iraq.
J Diabetes Metab Disord. 2021 Jan 21;20(1):237-243. doi: 10.1007/s40200-021-00738-x. eCollection 2021 Jun.
diabetes mellitus (type 2) is a chronic disease characterized by hyperglycemia resulting either from a deficiency of insulin, or decrease insulin action or both. This study focused on determining the new marker F- box and WD repeat domain containing protein 7 (FBXW7) as well as Fetuin -A and their correlation with other parameters in Diabetes mellitus type 2.
Sixty diabetes type2 patients were recruited from (National diabetes Center for treatment and Research) at Al Mustansriya University in Iraq during the period (from November 2018-March 2019) with an age range (37-69) years. The patient group was subdivided into two groups: group I (30) newly diagnosed T2DM and group II (30) T2DM (on metformin monotherapy), for comparison, 30 healthy individuals were included as a control. The protein levels (FBXW7, and Fetuin-A) were estimated using quantitative enzyme-linked immunesorbent assay (ELISA). Biochemical parameters; fasting serum glucose (FSG), and lipid profile were measured through spectrophotometrically. Insulin was determined using ELISA kit finally, HbA1c was determined using HPLC.
Serum levels of FBXW7 protein were significantly higher (p-value = 0.01), whereas, there was no significant difference (p ˃ 0.05) in the level of Fetuin-A in group I as compared to control group. There was no significant difference (p ˃ 0.05) in the levels of both proteins who group II was compared with the control group. On the other hand, FBXW7 level were significantly lower in group II as compared to group I. Fasting blood sugar FSG, glycated hemoglobin HbA1c, body mass index BMI, waist- to-height ratio WHtR, and lipid profile were significantly elevated, except for high density lipoproteins, HDL was decreased in Group I as compared to control group, whereas insulin appeared non-significant (P > 0.05). FBXW7 Protein was negatively correlated to Fetuin -A, HOMA-IR, Insulin concentration, and BMI, whereas positive correlate to FSG, HbA1c, lipid profile, and atherogenic index. According to ROC analysis, the optimal cutoff value for FBXW7 protein was (1.455) ng/ml.
Levels of FBXW7 and Fetuin-A proteins in sera of Diabetic patients were elevated. FBXW7 was significantly correlated with newly diagnosed patients. Metformin reduces FBXW7 and Fetuin-A levels.
2型糖尿病是一种以高血糖为特征的慢性疾病，其病因要么是胰岛素缺乏，要么是胰岛素作用降低，或者两者兼而有之。本研究着重于确定新的标志物含F-盒和WD重复结构域蛋白7（FBXW7）以及胎球蛋白-A，及其与2型糖尿病其他参数的相关性。
2018年11月至2019年3月期间，从伊拉克穆斯坦西里亚大学的（国家糖尿病治疗与研究中心）招募了60例2型糖尿病患者，年龄范围为37至69岁。患者组分为两组：第一组（30例）为新诊断的2型糖尿病患者，第二组（30例）为接受二甲双胍单药治疗的2型糖尿病患者，作为对照，纳入30名健康个体。使用定量酶联免疫吸附测定（ELISA）法估计蛋白质水平（FBXW7和胎球蛋白-A）。通过分光光度法测量生化参数；空腹血糖（FSG）和血脂谱。最后使用ELISA试剂盒测定胰岛素，使用高效液相色谱法测定糖化血红蛋白（HbA1c）。
FBXW7蛋白的血清水平显著更高（p值=0.01），而与对照组相比，第一组胎球蛋白-A水平无显著差异（p＞0.05）。第二组与对照组相比，两种蛋白质的水平均无显著差异（p＞0.05）。另一方面，与第一组相比，第二组FBXW7水平显著更低。空腹血糖（FSG）、糖化血红蛋白（HbA1c）、体重指数（BMI）、腰高比（WHtR）和血脂谱显著升高，除高密度脂蛋白外，与对照组相比，第一组高密度脂蛋白（HDL）降低，而胰岛素水平无显著差异（P＞0.05）。FBXW7蛋白与胎球蛋白-A、胰岛素抵抗指数（HOMA-IR）、胰岛素浓度和BMI呈负相关，而与FSG、HbA1c、血脂谱和动脉粥样硬化指数呈正相关。根据ROC分析，FBXW7蛋白的最佳截断值为1.455 ng/ml。
糖尿病患者血清中FBXW7和胎球蛋白-A蛋白水平升高。FBXW7与新诊断患者显著相关。二甲双胍可降低FBXW7和胎球蛋白-A水平。
