Nguyen Ngoc-Lan, Van Dung Vu, Van Tung Nguyen, Nguyen Thi Kim Lien, Quan Nguyen Duc, Giang Tran Thi Huong, Ngan Nguyen Thi Thanh, Hien Nguyen Thanh, Nguyen Huy-Hoang
Institute of Genome Research, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi Vietnam.
Graduate of Science and Technology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi Vietnam.
3 Biotech. 2023 Dec;13(12):416. doi: 10.1007/s13205-023-03833-3. Epub 2023 Nov 24.
In this study, strain DM10 was isolated from mangrove roots and characterized as a halotolerant plant growth-promoting bacterium. Strain DM10 exhibited the ability to solubilize phosphate, produce siderophore, show 1-aminocyclopropane-1-carboxylic acid deaminase activity, and hydrolyze starch. The rice plants subjected to a treatment of NaCl (200 mM) and inoculated with strain DM10 showed an improvement in the shoot length, root length, and dried weight, when compared to those exposed solely to saline treatment. The comprehensive genome sequencing of strain DM10 revealed a genome spanning of 4,171,745 bp, harboring 3626 protein coding sequences. Within its genome, strain DM10 possesses genes responsible for both salt-in and salt-out strategies, indicative of a robust genetic adaptation aimed at fostering salt tolerance. Additionally, the genome encodes genes involved in phosphate solubilization, such as the synthesis of gluconic acid, high-affinity phosphate transport systems, and alkaline phosphatase. In the genome of DM10, we identified the gene, responsible for encoding 1-aminocyclopropane-1-carboxylate deaminase, as well as the gene, which encodes α-amylase. Furthermore, the genome of DM10 contains sequences associated with the iron (3)-hydroxamate and iron uptake clusters, responsible for siderophore production. Such data provide a deep understanding of the mechanism employed by strain DM10 to combat osmotic and salinity stress, facilitate plant growth, and elucidate its molecular-level behaviors.
在本研究中，菌株DM10从红树林根部分离得到，并被鉴定为一种耐盐促植物生长细菌。菌株DM10具有溶解磷酸盐、产生铁载体、表现出1-氨基环丙烷-1-羧酸脱氨酶活性以及水解淀粉的能力。与仅接受盐处理的水稻植株相比，经200 mM NaCl处理并接种菌株DM10的水稻植株在地上部长度、根长度和干重方面均有所改善。菌株DM10的全基因组测序显示其基因组跨度为4,171,745 bp，含有3,626个蛋白质编码序列。在其基因组中，菌株DM10拥有负责盐进和盐出策略的基因，这表明其具有强大的遗传适应性以增强耐盐性。此外，该基因组编码参与磷酸盐溶解的基因，如葡萄糖酸的合成、高亲和力磷酸盐转运系统和碱性磷酸酶。在DM10的基因组中，我们鉴定出负责编码1-氨基环丙烷-1-羧酸脱氨酶的基因以及编码α-淀粉酶的基因。此外，DM10的基因组包含与铁（III）-异羟肟酸和铁摄取簇相关的序列，这些序列负责铁载体的产生。这些数据为深入了解菌株DM10对抗渗透和盐胁迫、促进植物生长的机制以及阐明其分子水平行为提供了依据。
