College of Public Health, University of South Florida, Tampa, FL, USA.
Division of Infectious Disease and International Medicine, Department of Internal Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL, USA.
Brief Bioinform. 2021 Mar 22;22(2):845-854. doi: 10.1093/bib/bbab004.
Humans have coexisted with pathogenic microorganisms throughout its history of evolution. We have never halted the exploration of pathogenic microorganisms. With the improvement of genome-sequencing technology and the continuous reduction of sequencing costs, an increasing number of complete genome sequences of pathogenic microorganisms have become available. Genome annotation of this massive sequence information has become a daunting task in biological research. This paper summarizes the approaches to the genome annotation of pathogenic microorganisms and the available popular genome annotation tools for prokaryotes, eukaryotes and viruses. Furthermore, real-world comparisons of different annotation tools using 12 genomes from prokaryotes, eukaryotes and viruses were conducted. Current challenges and problems were also discussed.
人类在其进化历史上一直与致病微生物共存。我们从未停止过对致病微生物的探索。随着基因组测序技术的提高和测序成本的不断降低，越来越多的致病微生物完整基因组序列得以问世。对这些海量序列信息进行基因组注释已成为生物研究中的一项艰巨任务。本文总结了致病微生物基因组注释的方法以及现有的用于原核生物、真核生物和病毒的流行基因组注释工具。此外，还使用来自原核生物、真核生物和病毒的 12 个基因组对不同注释工具进行了实际比较。同时还讨论了当前面临的挑战和问题。
