Department of Pharmacy, Faculty of Medicine, University of Niš, Blvd. Dr Zorana Đinđica 81, 18000, Niš, Serbia.
High School "Bora Stanković", Vožda Karađorđa 27, 18000, Niš, Serbia.
Chem Biodivers. 2024 Feb;21(2):e202301418. doi: 10.1002/cbdv.202301418. Epub 2023 Dec 5.
The objective of the present study was to assess the difference in antimicrobial activity of Satureja kitaibelii Wierzb. ex Heuff. essential oil at three growth stages. In addition, using statistical and chemometric tools, we tried to explain why the essential oil from a certain vegetation stage shows the highest antimicrobial activity. S. kitaibelii essential oils demonstrated minimum inhibitory concentration values from 160 to 10000 μg mL , and minimum microbicidal concentration values from 630 to 20000 μg mL . Geraniol, borneol, limonene and p-cymene are the dominant compounds of S. kitaibelii essential oil. The most abundant compound, geraniol, possesses antimicrobial activity in a range of MIC values from 40 to 5000 μg mL and MMC values from 80 to 10000 μg mL . The highest activity of essential oil for all tested strains of microorganisms was recorded in November. Results of statistical analysis indicate that the percentage of dominant compounds of essential oils does not affect the antibacterial activity of essential oils. Chemometric analyses leads to the conclusion that borneol, spathulenol, caryophyllene oxide and limonene can be the main contributors to the antibacterial activity of essential oil from November and that their mutual ratio is important. These results may represent a new methodological approach for future research on essential oils.
本研究的目的是评估在三个生长阶段中，Kitaibel 牛至精油的抗菌活性差异。此外，我们还使用统计和化学计量学工具，试图解释为什么某些生长阶段的精油表现出最高的抗菌活性。S. kitaibelii 精油的最小抑菌浓度值为 160-10000μg/mL，最小杀菌浓度值为 630-20000μg/mL。香叶醇、龙脑、柠檬烯和对伞花烃是 K. kitaibelii 精油的主要成分。含量最多的化合物香叶醇在 MIC 值为 40-5000μg/mL 和 MMC 值为 80-10000μg/mL 的范围内具有抗菌活性。对于所有测试的微生物菌株，精油的最高活性都记录在 11 月。统计分析的结果表明，精油中主要化合物的百分比并不影响精油的抗菌活性。化学计量学分析得出的结论是，龙脑、斯巴醇、石竹烯氧化物和柠檬烯可能是 11 月精油具有抗菌活性的主要贡献者，它们之间的相互比例很重要。这些结果可能代表了未来对精油进行研究的一种新的方法学方法。
