Phytochemical components and antibacterial activity of two populations of L. essential oils as traditional medicine plant.

BACKGROUND AND OBJECTIVES

L., a member of the Asteraceae family, has been widely employed in traditional Iranian herbal practices for centuries. This research seeks to analyze and compare the essential oil compositions and antibacterial characteristics of two distinct populations of

MATERIALS AND METHODS

Essential oils were obtained from the above-ground parts of these populations through hydrodistillation, and their chemical constituents were examined using gas chromatography-mass spectrometry. The antibacterial effectiveness of the essential oils against both Gram-positive and Gram-negative bacteria was evaluated employing the agar well diffusion technique.

RESULTS

Monoterpene hydrocarbons were found to be dominated in both populations, with Humulene epoxide II being the primary constituent, constituting 17.87% in the first population and 21.55% in the second one. The agar-well diffusion method revealed significant antibacterial effects of the essential oils. The findings indicated that the essential oil displayed heightened activity against in both populations. Furthermore, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests indicated that with concentrations of 400 μg/mL for both tests, was the most susceptible bacteria, while with MIC = 800 and MBC>800 μg/mL was the most resistant in both populations of

CONCLUSION

This research highlights the significance of as a valuable reservoir of monoterpene-rich oil exhibiting robust antibacterial characteristics, suggesting its potential use in the development of novel and naturally derived therapeutics for bacterial diseases.