Rand M L, Hennissen A A, Hornstra G
Atherosclerosis. 1986 Dec;62(3):267-76. doi: 10.1016/0021-9150(86)90101-2.
The relationships among platelet membrane fluidity, arterial thrombosis tendency, and platelet aggregation and prostanoid formation were investigated after feeding rats diets enriched in (n-6) or (n-3) polyunsaturated fatty acids. For at least 8 weeks, rats were fed a control diet containing 5 energy % (en%) sunflowerseed oil, a diet high in sunflowerseed oil (50 en%) which contains large amounts of 18:2(n-6), or a diet high in marine oil (5 en% sunflowerseed oil plus 45 en% sperm-whale oil) which contains considerable amounts of 20:5(n-3) and its desaturation and elongation products. Compared with the control diet, platelet membrane fluidity, measured by fluorescence polarization using platelets labelled with 1,6-diphenyl-1,3,5-hexatriene, was significantly increased upon feeding of the sunflowerseed-oil diet or the marine-oil diet. Both of these diets lowered arterial thrombosis tendency, measured by Hornstra's loop technique. Aggregation of platelets in whole blood activated with collagen tended to be higher upon sunflowerseed-oil feeding and lower in the marine-oil group. The concomitant formation of thromboxane A2 was not altered in the sunflowerseed-oil group, but was significantly reduced upon feeding the marine-oil diet. The results indicate that an increase in platelet membrane fluidity may be associated with a reduction in arterial thrombosis tendency. With the techniques used, no direct relationship could be observed among platelet membrane fluidity, platelet aggregation and platelet prostanoid production.
在给大鼠喂食富含（n-6）或（n-3）多不饱和脂肪酸的饮食后，研究了血小板膜流动性、动脉血栓形成倾向与血小板聚集及前列腺素生成之间的关系。至少8周内，给大鼠喂食含5%能量（en%）向日葵油的对照饮食、富含向日葵油（50 en%）且含有大量18:2（n-6）的饮食或富含海洋油（5 en%向日葵油加45 en%抹香鲸油）且含有大量20:5（n-3）及其去饱和和延长产物的饮食。与对照饮食相比，用1,6-二苯基-1,3,5-己三烯标记血小板通过荧光偏振测量的血小板膜流动性，在喂食向日葵油饮食或海洋油饮食后显著增加。这两种饮食均降低了用霍恩斯特拉环技术测量的动脉血栓形成倾向。用胶原蛋白激活的全血中血小板聚集在喂食向日葵油时往往较高，而在海洋油组中较低。血栓素A2的伴随生成在向日葵油组中未改变，但在喂食海洋油饮食后显著降低。结果表明，血小板膜流动性增加可能与动脉血栓形成倾向降低有关。使用这些技术，未观察到血小板膜流动性、血小板聚集和血小板前列腺素生成之间的直接关系。
