miR-186-5p、miR-125b-5p 和 miR-1260a 在软骨肉瘤中的功能和调控
Function and regulation of miR-186-5p, miR-125b-5p and miR-1260a in chordoma.
机构信息
Department of Neurosurgery, Beijing Tiantan Hospital, Capital Medical University, Nansihuanxilu 119, Fengtai District, Beijing, 100070, China.
China National Clinical Research Center for Neurological Diseases, Beijing, China.
出版信息
BMC Cancer. 2023 Nov 27;23(1):1152. doi: 10.1186/s12885-023-11238-x.
BACKGROUND
The function and regulation of miRNAs in progression of chordoma were unclear.
METHODS
Five miRNAs were identified by the machine learning method from the miRNA expression array. CCk-8 assay, EDU assay, wound healing migration assay, and trans-well assay were used to reveal the effect of the miRNAs in chordoma cell lines. Moreover, bioinformation analysis and the mRNA expression array between the primary chordomas and recurrent chordomas were used to find the target protein genes of miRNAs. Furthermore, qRT-PCR and luciferase reporter assay were used to verify the result.
RESULTS
miR-186-5p, miR-30c-5p, miR-151b, and miR-125b-5p could inhibit proliferation, migration, and invasion of chordoma while miR-1260a enhances proliferation, migration, and invasion of chordoma. Recurrent chordoma has a worse disease-free outcome than the primary chordoma patients. AMOT, NPTX1, RYR3, and P2RX5 were the target protein mRNAs of miR-186-5p; NPTX1 was the target protein mRNAs of miR-125b-5p; and AMOT and TNFSF14 were the target protein mRNAs of miR-1260a.
CONCLUSIONS
miR-186-5p, miR-125b-5p, miR-1260a, and their target protein mRNAs including AMOT, NPTX1, RYR3, P2RX5, TNFSF14 may be the basement of chordoma research.
背景
miRNA 在脊索瘤进展中的功能和调节作用尚不清楚。
方法
通过机器学习方法从 miRNA 表达谱中鉴定了 5 个 miRNA。CCk-8 检测、EDU 检测、划痕愈合迁移检测和 Transwell 检测用于揭示 miRNA 对脊索瘤细胞系的影响。此外，生物信息分析和原发性脊索瘤与复发性脊索瘤之间的 mRNA 表达谱用于寻找 miRNA 的靶蛋白基因。进一步通过 qRT-PCR 和荧光素酶报告基因检测验证结果。
结果
miR-186-5p、miR-30c-5p、miR-151b 和 miR-125b-5p 可抑制脊索瘤的增殖、迁移和侵袭，而 miR-1260a 则增强脊索瘤的增殖、迁移和侵袭。复发性脊索瘤患者的无病生存期较原发性脊索瘤患者差。AMOT、NPTX1、RYR3 和 P2RX5 是 miR-186-5p 的靶蛋白 mRNAs；NPTX1 是 miR-125b-5p 的靶蛋白 mRNAs；AMOT 和 TNFSF14 是 miR-1260a 的靶蛋白 mRNAs。
结论
miR-186-5p、miR-125b-5p、miR-1260a 及其靶蛋白 mRNAs，包括 AMOT、NPTX1、RYR3、P2RX5 和 TNFSF14，可能是脊索瘤研究的基础。
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