Department of Dental Materials and Prosthodontics, Ribeirão Preto School of Dentistry, University of São Paulo, Ribeirão Preto, Brazil.
Department of Pediatric Dentistry, Ribeirão Preto School of Dentistry, University of São Paulo, Ribeirão Preto, Brazil.
J Esthet Restor Dent. 2024 May;36(5):770-777. doi: 10.1111/jerd.13172. Epub 2023 Nov 28.
This study evaluated the effect of bromelain and propolis extract on the bond strength (BS) of a universal adhesive system to eroded dentin.
Sixty human molars with exposed dentin were halved, with one half protected by composite resin and the other subjected to erosive treatment followed by remineralization. After the erosive treatment, the composite resin was removed, and the teeth were randomly assigned to three groups (n = 20): Adhesive-Control System; Br-10%; Pr-16%. Following the treatments, composite resin blocks were built on the dentin surfaces and sticks of 0.9 mm2 were obtained and stored in distilled water at 37°C for 24 h and 6 months. After these periods, the sticks underwent bond strength testing and the data were analyzed using 2-way ANOVA, Bonferroni test, p < 0.05. Fracture patterns were observed using light microscope and scanning electron microscopy.
Irrespective of the substrate and aging duration, propolis demonstrated higher BS (p < 0.05) compared to the other treatments. Eroded dentin exhibited greater removal of the smear layer and dentinal tubules with a larger diameter than sound dentin, especially when treated with bromelain, resulting in the formation of resin tags.
Propolis consistently promoted the highest bond strength, irrespective of aging or substrate. Eroded dentin treated with propolis, or bromelain exhibited a higher prevalence of non-adhesive fractures and resin tag formation.
Propolis shows promise for enhancing the longevity of adhesive restorations in eroded dentin due to its ability to promote high bond strength.
本研究评估菠萝蛋白酶和蜂胶提取物对一种通用粘结剂系统在酸蚀牙本质粘结强度（BS）的影响。
60 颗暴露牙本质的人磨牙被对半切开，其中一半用复合树脂保护，另一半进行酸蚀处理和再矿化。酸蚀处理后，去除复合树脂，将牙齿随机分为三组（n=20）：粘结对照组；Br-10%；Pr-16%。处理后，将复合树脂块构建在牙本质表面，获得 0.9mm2 的棒状试件，并在 37°C的蒸馏水中储存 24h 和 6 个月。在这两个时间段后，对棒状试件进行粘结强度测试，并使用双因素方差分析、Bonferroni 检验进行数据分析，p<0.05。使用光显微镜和扫描电子显微镜观察断裂模式。
无论基底和老化时间如何，蜂胶的粘结强度（BS）均高于其他处理（p<0.05）。与正常牙本质相比，酸蚀牙本质的玷污层和牙本质小管去除更多，直径更大，特别是用菠萝蛋白酶处理后，形成了树脂突。
无论老化或基底如何，蜂胶均能持续提高粘结强度。用蜂胶或菠萝蛋白酶处理的酸蚀牙本质更易发生非粘结性断裂和树脂突形成。
由于能够提高粘结强度，蜂胶有望延长酸蚀牙本质粘结修复的寿命。
