Department of Dental Materials and Prosthodontics, Ribeirão Preto School of Dentistry, University of São Paulo, Av do Cafe, s/nº, Ribeirão Preto, Sao Paulo, 14040-904, Brazil.
University of Ribeirao Preto, Av. Costábile Romano, 2201 - Nova Ribeirânia, Ribeirão Preto, Sao Paulo, 14096-900, Brazil.
J Dent. 2021 Mar;106:103585. doi: 10.1016/j.jdent.2021.103585. Epub 2021 Jan 16.
This study evaluated the effect of propolis associated with Biosilicate on the bond strength (BS) and gelatinolytic activity at the adhesive/dentin interface.
Occlusal cavities were prepared in 320 human molars. Half of them were submitted to cariogenic challenge. All the teeth were separated into eight groups (n = 20): Control - Adhesive System (Single Bond Universal, 3 MESPE); CHX - 0.12 % Chlorhexidine; Bio - 10 % Biosilicate; P16 - Propolis with low levels of polyphenols; P45 - Propolis with high levels of polyphenols; CHX Bio - CHX + Bio; P16 Bio - P16+Bio; P45 Bio - P45+Bio. The adhesive was applied (self-etch mode) after treatments. Restorations (Filtek Z350, 3 MESPE) were performed and samples sectioned into sticks, separated and stored in distilled water at 37 °C for 24 h, 6 months and 1 year. Microtensile BS (0.5 mm/min) was tested and analyzed (2-way ANOVA, Bonferroni's Test, p < .05 and Weibull analysis). Fracture patterns (VH-M100, Keyence) and adhesive interfaces (SEM, EVO-MA10, ZEISS and TEM, JEM-1010, JEOL) were observed; and biodegradation and in situ zymography performed.
P16 presented the highest BS values on sound dentin after 6 months. In caries-affected dentin (CAD), the association of treatments promoted the highest BS after 24 h. Sound dentin obtained significantly higher Weibull modulus than CAD. SEM displayed resin tags in P16, P45 and association of treatments. TEM showed good interaction between adhesive and dentin. According to the in situ zymography and biodegradation assay all natural primers reduced the gelatinolytic activity. P45 presented the lowest biodegradation and enzymatic activity.
Propolis and the association of treatments promoted the highest bond strength results and preserved the dentin. All the experimental groups exhibited low gelatinolytic activity.
Propolis and the association of treatments with Biosilicate could preserve the dentin substrate and improve the longevity of composite restorations.
本研究评估蜂胶联合 Biosilicate 对牙本质粘结强度（BS）和胶溶解活性的影响。
在 320 个人类磨牙的牙合面制备窝洞。其中一半进行致龋挑战。所有牙齿分为八组（n = 20）：对照组 - 粘接系统（Single Bond Universal，3 MESPE）；CHX - 0.12%洗必泰；Bio - 10% Biosilicate；P16 - 低多酚水平的蜂胶；P45 - 高多酚水平的蜂胶；CHX Bio - CHX+Biosilicate；P16 Bio - P16+Biosilicate；P45 Bio - P45+Biosilicate。处理后应用粘接剂（自酸蚀模式）。进行修复（Filtek Z350，3 MESPE），并将样本切成棒状，分离并在 37°C 的蒸馏水中储存 24 h、6 个月和 1 年。测试并分析微拉伸 BS（0.5 mm/min）（2 因素方差分析、Bonferroni 检验，p<.05 和威布尔分析）。观察断裂模式（VH-M100，Keyence）和粘接界面（SEM，EVO-MA10，ZEISS 和 TEM，JEM-1010，JEOL）；进行生物降解和原位酶谱分析。
P16 在 6 个月时对正常牙本质的 BS 值最高。在龋损牙本质（CAD）中，治疗联合应用在 24 h 时可获得最高的 BS。正常牙本质的威布尔模数明显高于 CAD。SEM 显示 P16、P45 和治疗联合应用中有树脂突。TEM 显示粘接剂与牙本质之间具有良好的相互作用。根据原位酶谱和生物降解试验，所有天然引物均降低了胶溶解活性。P45 的生物降解和酶活性最低。
蜂胶联合治疗可提高牙本质的粘结强度，保护牙本质。所有实验组的胶溶解活性均较低。
蜂胶联合 Biosilicate 治疗可保护牙本质基底，提高复合树脂修复的耐久性。
