马立克氏病病毒的 LORF9 参与 B 淋巴细胞的早期裂解复制，并且可以作为基因缺失疫苗开发的靶标。
LORF9 of Marek's disease virus is involved in the early cytolytic replication of B lymphocytes and can act as a target for gene deletion vaccine development.
机构信息
College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, China.
International Joint Research Center of National Animal Immunology, Henan Agricultural University, Zhengzhou, China.
出版信息
J Virol. 2023 Dec 21;97(12):e0157423. doi: 10.1128/jvi.01574-23. Epub 2023 Nov 28.
Abstract
Marek's disease virus (MDV) is a highly infectious and oncogenic virus that can induce severe T cell lymphomas in chickens. MDV encodes more than 100 genes, most of which have unknown functions. This work indicated that the gene is necessary for MDV early cytolytic replication in B lymphocytes. In addition, we have found that the deletion mutant has a comparative immunological protective effect with CVI988/Rispens vaccine strain against very virulent MDV challenge. This is a significant discovery that LORF9 can be exploited as a possible target for the development of an MDV gene deletion vaccine.
摘要
马立克氏病病毒（MDV）是一种高度传染性和致癌性的病毒，可在鸡中诱导严重的 T 细胞淋巴瘤。MDV 编码 100 多个基因，其中大多数功能未知。这项工作表明，该基因对于 MDV 在 B 淋巴细胞中的早期细胞裂解复制是必需的。此外，我们发现，与 CVI988/Rispens 疫苗株相比， 缺失突变体对超强毒力 MDV 攻击具有相当的免疫保护作用。这是一项重要的发现，LORF9 可以作为开发 MDV 基因缺失疫苗的潜在靶点。
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