College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, Henan, People's Republic of China.
International Joint Research Center of National Animal Immunology, College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, Henan, People's Republic of China.
Sci Rep. 2021 May 26;11(1):11084. doi: 10.1038/s41598-021-90548-1.
Marek's disease virus (MDV) induces severe immunosuppression and lymphomagenesis in the chicken, its natural host, and results in a condition that investigated the pathogenesis of MDV and have begun to focus on the expression profiling of circular RNAs (circRNAs). However, little is known about how the expression of circRNAs is referred to as Marek's disease. Previous reports have is regulated during MDV replication. Here, we carried out a comprehensive profiling analysis of N6-methyladenosine (mA) modification on the circRNA transcriptome in infected and uninfected chicken embryonic fibroblast (CEF) cells. Methylated RNA immunoprecipitation sequencing (MeRIP-Seq) revealed that mA modification was highly conserved in circRNAs. Comparing to the uninfected group, the number of peaks and conserved motifs were not significantly different in cells that were infected with MDV, although reduced abundance of circRNA mA modifications. However, gene ontology and Kyoto encyclopedia of genes and genomes (KEGG) pathway analyses revealed that the insulin signaling pathway was associated with the regulation of mA modified circRNAs in MDV infection. This is the first report to describe alterations in the transcriptome-wide profiling of mA modified circRNAs in MDV-infected CEF cells.
马立克氏病病毒（MDV）在其自然宿主鸡中诱导严重的免疫抑制和淋巴瘤发生，并导致对 MDV 发病机制的研究，并开始关注环状 RNA（circRNA）的表达谱分析。然而，人们对 circRNA 的表达如何被调控知之甚少。此前的研究报告表明，circRNA 的表达在 MDV 复制过程中受到调节。在这里，我们对感染和未感染鸡胚胎成纤维细胞（CEF）细胞中的 circRNA 转录组的 N6-甲基腺苷（m6A）修饰进行了全面的分析。甲基化 RNA 免疫沉淀测序（MeRIP-Seq）表明，m6A 修饰在 circRNA 中高度保守。与未感染组相比，感染 MDV 的细胞中峰的数量和保守基序没有显著差异，尽管 circRNA m6A 修饰的丰度降低。然而，基因本体论和京都基因与基因组百科全书（KEGG）通路分析表明，胰岛素信号通路与 MDV 感染中 m6A 修饰的 circRNA 调节有关。这是第一篇描述 MDV 感染的 CEF 细胞中全转录组范围的 m6A 修饰 circRNA 谱变化的报告。
