Department of Internal Medicine, Division of Infectious Diseases and Tropical Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
Department of Infectious Diseases, Chi Mei Medical Center, Tainan, Taiwan.
mSphere. 2023 Dec 20;8(6):e0040023. doi: 10.1128/msphere.00400-23. Epub 2023 Nov 28.
, a Gram-negative pathogen, causes infections such as bacteraemia, pneumonia, and neonatal meningitis. The pathogen resists most antimicrobial classes, making novel approaches urgently needed. In natural settings, Gram-negative bacteria secrete outer membrane vesicles (OMVs) that carry important molecules in the bacterial life cycle. These OMVs are enriched with proteins involved in virulence, survival, and carbohydrate metabolism, making them a promising source for vaccine development against the pathogen. This study investigated the efficacy of imipenem-induced OMVs (iOMVs) as a vaccine candidate against infection in a mouse pneumonia model. Mice immunized with iOMVs were completely protected during lethal-dose challenges. Passive immunization with hyperimmune sera and splenocytes conferred protection against lethal pneumonia. Further investigation is needed to understand the mechanisms underlying the protective effects of iOMV-induced passive immunity, such as the action on specific antibody subclasses or T cell subsets.
铜绿假单胞菌是一种革兰氏阴性病原体，可引起菌血症、肺炎和新生儿脑膜炎等感染。该病原体对大多数抗菌药物类别具有耐药性，因此迫切需要新的方法。在自然环境中，革兰氏阴性细菌会分泌外膜囊泡（OMVs），其中包含细菌生命周期中的重要分子。这些 OMVs 富含与毒力、存活和碳水化合物代谢相关的蛋白质，因此它们是开发针对该病原体的疫苗的有前途的来源。本研究在小鼠肺炎模型中调查了亚胺培南诱导的 OMVs（iOMVs）作为疫苗候选物对抗 感染的功效。用 iOMVs 免疫的小鼠在致死剂量挑战期间完全受到保护。用高免血清和脾细胞进行被动免疫可预防致死性肺炎。需要进一步研究来了解 iOMV 诱导的被动免疫的保护作用的机制，例如针对特定抗体亚类或 T 细胞亚群的作用。
