全基因组测序揭示两成分系统基因突变与结核分枝杆菌传播的关系。

Association between two-component systems gene mutation and Mycobacterium tuberculosis transmission revealed by whole genome sequencing.

机构信息

Shandong University of Traditional Chinese Medicine, Jinan, Shandong, 250014, People's Republic of China.

Artificial Intelligence Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, Shandong, 250011, People's Republic of China.

出版信息

BMC Genomics. 2023 Nov 28;24(1):718. doi: 10.1186/s12864-023-09788-2.

DOI:10.1186/s12864-023-09788-2

PMID:38017383

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10683263/

Abstract

BACKGROUND

Two-component systems (TCSs) assume a pivotal function in Mycobacterium tuberculosis (M.tuberculosis) growth. However, the exact regulatory mechanism of this system needs to be elucidated, and only a few studies have investigated the effect of gene mutations within TCSs on M.tuberculosis transmission. This research explored the relationship between TCSs gene mutation and the global transmission of (M.tuberculosis).

RESULTS

A total of 13531 M.tuberculosis strains were enrolled in the study. Most of the M.tuberculosis strains belonged to lineage4 (n=6497,48.0%), followed by lineage2 (n=5136,38.0%). Our results showed that a total of 36 single nucleotide polymorphisms (SNPs) were positively correlated with clustering of lineage2, such as Rv0758 (phoR, C820G), Rv1747(T1102C), and Rv1057(C1168T). A total of 30 SNPs showed positive correlation with clustering of lineage4, such as phoR(C182A, C1184G, C662T, T758G), Rv3764c (tcrY, G1151T), and Rv1747 C20T. A total of 19 SNPs were positively correlated with cross-country transmission of lineage2, such as phoR A575C, Rv1028c (kdpD, G383T, G1246C), and Rv1057 G817T. A total of 41 SNPs were positively correlated with cross-country transmission of lineage4, such as phoR(T758G, T327G, C284G), kdpD(G1755A, G625C), Rv1057 C980T, and Rv1747 T373G.

CONCLUSIONS

Our study identified that SNPs in genes of two-component systems were related to the transmission of M. tuberculosis. This finding adds another layer of complexity to M. tuberculosis virulence and provides insight into future research that will help to elucidate a novel mechanism of M. tuberculosis pathogenicity.

摘要

背景

双组分系统（TCSs）在结核分枝杆菌（M.tuberculosis）的生长中起着至关重要的作用。然而，该系统的确切调控机制仍需阐明，并且只有少数研究调查了 TCS 内基因突变对 M.tuberculosis 传播的影响。本研究探讨了 TCS 基因突变与（M.tuberculosis）全球传播之间的关系。

结果

本研究共纳入了 13531 株 M.tuberculosis 株。大多数 M.tuberculosis 株属于谱系 4（n=6497，48.0%），其次是谱系 2（n=5136，38.0%）。我们的结果表明，共有 36 个单核苷酸多态性（SNPs）与谱系 2 的聚类呈正相关，例如 Rv0758（phoR，C820G）、Rv1747（T1102C）和 Rv1057（C1168T）。共有 30 个 SNPs 与谱系 4 的聚类呈正相关，例如 phoR（C182A、C1184G、C662T、T758G）、Rv3764c（tcrY，G1151T）和 Rv1747 C20T。共有 19 个 SNPs 与谱系 2 的跨国传播呈正相关，例如 phoR A575C、Rv1028c（kdpD，G383T，G1246C）和 Rv1057 G817T。共有 41 个 SNPs 与谱系 4 的跨国传播呈正相关，例如 phoR（T758G、T327G、C284G）、kdpD（G1755A、G625C）、Rv1057 C980T 和 Rv1747 T373G。