Key Laboratory of Food Quality and Safety of Jiangsu Province, State Key Laboratory Breeding Base, Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Viruses. 2021 Aug 10;13(8):1576. doi: 10.3390/v13081576.
The small brown planthopper, (Fallén), is an important agricultural pest that causes significant losses by sucking and transmitting multiple plant viruses, such as rice black-streaked dwarf virus (RBSDV). Insecticides are commonly used to control planthoppers and cause the induction or overexpression of cytochrome P450 monooxygenases (P450s) from the CYP3 and CYP4 clades after insecticide application. However, little is known about the roles of insecticides and P450s in the regulation of viral replication in insects. In this study, RBSDV-infected were injected with imidacloprid, deltamethrin, pymetrozine, and buprofezin, respectively. The insecticide treatments caused a significant decrease in RBSDV abundance in . Treatment of piperonyl butoxide (PBO), an effective inhibitor of P450s, significantly increased the RBSDV abundance in . Fourteen P450 candidate genes in the CYP3 clade and 21 in the CYP4 clade were systematically identified in , and their expression patterns were analyzed under RBSDV infection, in different tissues, and at different developmental stages. Among the thirty-five P450 genes, the expression level of was the highest, while was the lowest after RBSDV infection. Knockdown of and significantly increased the virus abundance and promoted virus replication in . Overall, our data reveal that and play a critical role in the regulation of virus replication in .
小褐飞虱（Fallén）是一种重要的农业害虫，通过吸食和传播多种植物病毒，如水稻黑条矮缩病毒（RBSDV），造成巨大损失。杀虫剂通常用于防治飞虱，并在施药后诱导或过度表达 CYP3 和 CYP4 族的细胞色素 P450 单加氧酶（P450s）。然而，对于杀虫剂和 P450s 在昆虫中调节病毒复制的作用知之甚少。在本研究中，分别用吡虫啉、溴氰菊酯、吡丙醚和噻虫嗪对感染 RBSDV 的小褐飞虱进行注射。杀虫剂处理导致 中 RBSDV 丰度显著降低。增效醚（PBO）处理，一种有效的 P450s 抑制剂，显著增加了 中 RBSDV 的丰度。在 中系统鉴定了 CYP3 族的 14 个 P450 候选基因和 CYP4 族的 21 个基因，并分析了它们在 RBSDV 感染、不同组织和不同发育阶段的表达模式。在 35 个 P450 基因中， 基因的表达水平最高，而在 RBSDV 感染后 基因的表达水平最低。 基因和 基因的敲低显著增加了病毒丰度，并促进了 在 中的复制。总体而言，我们的数据表明 基因和 基因在调节 中病毒复制中起关键作用。
