School of Clinical Medicine, Ningxia Medical University, Yinchuan, China.
Key Laboratory of Common Infectious Disease Prevention and Control in Ningxia, Yinchuan, China.
Immun Inflamm Dis. 2023 Nov;11(11):e1082. doi: 10.1002/iid3.1082.
Cystic echinococcosis (CE), a zoonotic parasitic disease caused by Echinococcus granulosus, remains a public health and socioeconomic issue worldwide, making its prevention and treatment of vital importance. The aim of this study was to investigate changes in the intestinal microbiota of mice immunized with three peptide vaccines based on the recombinant antigen of E. granulosus, P29 (rEg.P29), with the hope of providing more valuable information for the development of vaccines against CE.
Three peptide vaccines, rEg.P29 , rEg.P29 , and rEg.P29 , were prepared based on rEg.P29, and a subcutaneous immunization model was established. The intestinal floras of mice in the different immunization groups were analyzed by 16 S rRNA gene sequencing.
The intestinal microbiota analysis at both immunization time points revealed that Firmicutes, Bacteroidota, and Verrucomicrobiota were the predominant flora at the phylum level, while at the genus level, Akkermansia, unclassified_Muribaculaceae, Lachnospiraceae_NK4A136_group, and uncultured_rumen bacterium were the dominant genera. Some probiotics in the intestines of mice were significantly increased after immunization with the peptide vaccines, such as Lactobacillus_taiwanensis, Lactobacillus_reuteri, Lachnospiraceae_NK4A136_group, Bacteroides_acidifaciens, and so forth. Meanwhile, some harmful or conditionally pathogenic bacteria were decreased, such as Turicibacter sanguinis, Desulfovibrio_fairfieldensis, Clostridium_sp, and so forth, most of which are associated with inflammatory or infectious diseases. Kyoto Encyclopaedia of Genes and Genomes enrichment analysis revealed that the differential flora were enriched in multiple metabolic pathways, primarily biological systems, human diseases, metabolism, cellular processes, and environmental information processing.
In this study, we comprehensively analyzed and compared changes in the intestinal microbiota of mice immunized with three peptide vaccines as well as their related metabolic pathways, providing a theoretical background for the development of novel vaccines against E. granulosus.
由细粒棘球绦虫（Echinococcus granulosus）引起的包虫病（cystic echinococcosis，CE）是一种人畜共患寄生虫病，仍然是全球公共卫生和社会经济问题，因此其预防和治疗至关重要。本研究旨在探讨基于细粒棘球绦虫重组抗原 P29（rEg.P29）的三种肽疫苗免疫小鼠后肠道微生物群的变化，以期为包虫病疫苗的开发提供更有价值的信息。
以 rEg.P29 为基础，制备三种肽疫苗 rEg.P29、rEg.P29 和 rEg.P29，并建立皮下免疫模型。采用 16S rRNA 基因测序分析不同免疫组小鼠的肠道菌群。
在两个免疫时间点的肠道微生物群分析中，厚壁菌门（Firmicutes）、拟杆菌门（Bacteroidota）和疣微菌门（Verrucomicrobiota）是优势菌群，而在属水平上，阿克曼氏菌（Akkermansia）、未分类的_Muribaculaceae、毛螺菌科_NK4A136 属群和未培养的瘤胃液菌（uncultured_rumen bacterium）是优势菌群。肽疫苗免疫后，小鼠肠道中一些益生菌显著增加，如台湾乳酸菌（Lactobacillus_taiwanensis）、雷氏乳杆菌（Lactobacillus_reuteri）、毛螺菌科_NK4A136 属群、拟杆菌属_产丁酸菌（Bacteroides_acidifaciens）等。同时，一些有害或条件致病细菌减少，如血链球菌（Turicibacter sanguinis）、费尔菲尔德脱硫弧菌（Desulfovibrio_fairfieldensis）、梭菌属（Clostridium_sp）等，其中大多数与炎症或感染性疾病有关。京都基因与基因组百科全书富集分析显示，差异菌群富集在多个代谢途径中，主要是生物系统、人类疾病、代谢、细胞过程和环境信息处理。
本研究全面分析和比较了三种肽疫苗免疫小鼠后肠道微生物群及其相关代谢途径的变化，为新型棘球蚴疫苗的开发提供了理论依据。
