Deshayes Samuel, Baugé Caroline, Dupont Pierre-Antoine, Simard Christophe, Rida Hanan, de Boysson Hubert, Manrique Alain, Aouba Achille
Department of Internal Medicine and Clinical Immunology, Normandie University, UNICAEN, CHU de Caen Normandie - Université Basse Normandie, Avenue de la Côte de Nacre, 14000, CAEN, France.
Normandie University, UNICAEN, CHU de Caen Normandie, UR4650 PSIR, Caen, France.
EJNMMI Res. 2023 Nov 29;13(1):103. doi: 10.1186/s13550-023-01039-5.
Metabolic imaging is routinely used to demonstrate aortitis in patients with giant-cell arteritis. We aimed to investigate the preclinical model of aortitis in BALB/c IL1rn mice using [F]fluorodeoxyglucose ([F]FDG) positron emission tomography-magnetic resonance (PET-MR), gamma counting and immunostaining. We used 15 first-generation specific and opportunistic pathogen-free (SOPF) 9-week-old IL1rn mice, 15 wild-type BALB/cAnN mice and 5 s-generation specific pathogen-free (SPF) 9-week-old IL1rn. Aortic [F]FDG uptake was assessed as the target-to-background ratio (TBR) using time-of-flight MR angiography as vascular landmarks.
[F]FDG uptake measured by PET or gamma counting was similar in the first-generation SOPF IL1rn mice and the wild-type group (p > 0.05). However, the first-generation IL1rn mice exhibited more interleukin-1β (p = 0.021)- and interleukin-6 (p = 0.019)-positive cells within the abdominal aorta than the wild-type mice. In addition, the second-generation SPF group exhibited significantly higher TBR (p = 0.0068) than the wild-type mice on the descending thoracic aorta, unlike the first-generation SOPF IL1rn mice.
In addition to the involvement of interleukin-1β and -6 in IL1rn mouse aortitis, this study seems to validate [F]FDG PET-MR as a useful tool for noninvasive monitoring of aortitis in this preclinical model.
代谢成像常用于显示巨细胞动脉炎患者的主动脉炎。我们旨在使用[F]氟脱氧葡萄糖（[F]FDG）正电子发射断层扫描-磁共振（PET-MR）、γ计数和免疫染色研究BALB/c IL1rn小鼠主动脉炎的临床前模型。我们使用了15只第一代特定无特定病原体（SOPF）9周龄的IL1rn小鼠、15只野生型BALB/cAnN小鼠和5只第二代特定病原体-free（SPF）9周龄的IL1rn小鼠。以飞行时间磁共振血管造影作为血管标志，将主动脉[F]FDG摄取评估为靶本底比（TBR）。
第一代SOPF IL1rn小鼠和野生型组通过PET或γ计数测量的[F]FDG摄取相似（p>0.05）。然而，第一代IL1rn小鼠腹主动脉内白细胞介素-1β（p=0.021）和白细胞介素-6（p=0.019）阳性细胞比野生型小鼠更多。此外，与第一代SOPF IL1rn小鼠不同，第二代SPF组在降主动脉的TBR显著高于野生型小鼠（p=0.0068）。
除白细胞介素-1β和-6参与IL1rn小鼠主动脉炎外，本研究似乎验证了[F]FDG PET-MR作为该临床前模型中主动脉炎无创监测的有用工具。
