INSERM, UMR_S 959, Inflammation-Immunopathology-Biotherapy Department, Sorbonne Université, UPMC University of Paris, Paris, France.
Department of Internal Medicine and Clinical Immunology, AP-HP, Groupe Hospitalier Pitié-Salpêtrière, Paris, France.
Sci Rep. 2021 Mar 15;11(1):5926. doi: 10.1038/s41598-021-84725-5.
Recent studies have provided evidence of a close link between specific microbiota and inflammatory disorders. While the vessel wall microbiota has been recently described in large vessel vasculitis (LVV) and controls, the blood microbiome in these diseases has not been previously reported (LVV). We aimed to analyse the blood microbiome profile of LVV patients (Takayasu's arteritis [TAK], giant cell arteritis [GCA]) and healthy blood donors (HD). We studied the blood samples of 13 patients with TAK (20 samples), 9 patients with GCA (11 samples) and 15 HD patients. We assessed the blood microbiome profile by sequencing the 16S rDNA blood bacterial DNA. We used linear discriminant analysis (LDA) coupled with linear discriminant effect size measurement (LEfSe) to investigate the differences in the blood microbiome profile between TAK and GCA patients. An increase in the levels of Clostridia, Cytophagia and Deltaproteobacteria and a decrease in Bacilli at the class level were found in TAK patients compared with HD patients (LDA > 2, p < 0.05). Active TAK patients had significantly lower levels of Staphylococcus compared with inactive TAK patients. Samples of GCA patients had an increased abundance of Rhodococcus and an unidentified member of the Cytophagaceae family. Microbiota of TAK compared with GCA patients was found to show higher levels of Candidatus Aquiluna and Cloacibacterium (LDA > 2; p < 0.05). Differences highlighted in the blood microbiome were also associated with a shift of bacterial predicted metabolic functions in TAK in comparison with HD. Similar results were also found in patients with active versus inactive TAK. In conclusion, patients with TAK were found to present a specific blood microbiome profile in comparison with healthy donors and GCA subjects. Significant changes in the blood microbiome profiles of TAK patients were associated with specific metabolic functions.
最近的研究提供了证据，证明特定的微生物群与炎症性疾病之间存在密切联系。虽然血管壁微生物群在大动脉炎（LVV）和对照中最近得到了描述，但这些疾病中的血液微生物组尚未被报道（LVV）。我们旨在分析 LVV 患者（大动脉炎 [TAK]、巨细胞动脉炎 [GCA]）和健康献血者（HD）的血液微生物组谱。我们研究了 13 例 TAK 患者（20 例）、9 例 GCA 患者（11 例）和 15 例 HD 患者的血液样本。我们通过测序 16S rDNA 血液细菌 DNA 来评估血液微生物组谱。我们使用线性判别分析（LDA）结合线性判别效应大小测量（LEfSe）来研究 TAK 和 GCA 患者血液微生物组谱之间的差异。与 HD 患者相比，TAK 患者的 Clostridia、Cytophagia 和 Deltaproteobacteria 水平升高，Bacilli 水平降低（LDA>2，p<0.05）。与静止性 TAK 患者相比，活动性 TAK 患者的金黄色葡萄球菌水平显著降低。GCA 患者的样本中，Rhodococcus 和未鉴定的 Cytophagaceae 家族成员的丰度增加。与 GCA 患者相比，TAK 患者的微生物群中发现了更高水平的 Candidatus Aquiluna 和 Cloacibacterium（LDA>2；p<0.05）。与 HD 相比，TAK 患者血液微生物组中突出的差异也与细菌预测代谢功能的转变有关。在活动性与静止性 TAK 患者中也发现了类似的结果。总之，与健康供体和 GCA 受试者相比，TAK 患者表现出特定的血液微生物组谱。TAK 患者血液微生物组谱的显著变化与特定的代谢功能有关。
