Department of Biochemistry, Karpagam Academy of Higher Education, Deemed to be University, Coimbatore, Tamil Nadu, 32, India.
Med Oncol. 2023 Nov 29;41(1):4. doi: 10.1007/s12032-023-02230-3.
Cancer is considered most detrimental due to high mortality worldwide. Among them, skin cancers play a major part by affecting one in three cancer patients globally. About 2-3 million cancer cases were reported to be non-melanoma and melanoma skin cancers, respectively. Although chemotherapeutic drugs act on cancer cells but results in long-lasting morbidities which affects one's quality of life and also works only in the initial stage of the cancer. Hence, an idea of traditional medicine to cure the disease efficiently with less side effects was pursued by the researchers. We have assessed the combination effect of p-coumaric acid and naringin in exerting anticancer activity using A431 (epidermoid carcinoma) cells. The MTT analysis of the combination on A431 cells showed the least IC concentration of 41 µg/ml which is effective than the standard drug imiquimod with IC concentration of 52 µg/ml. Further, flow cytometric analysis was carried out to identify the molecular mechanism behind the anticancer effects of the combination. The results revealed that the combination arrested the A431 cell cycle at S phase, induced apoptosis as indicated by more early and late apoptotic cells when compared with the control, and further altered reactive oxygen species (ROS) and mitochondrial membrane potential in A431 cells. Hence, the results suggest the potential anticancer effects of p-coumaric acid and naringin combination against the skin cancer (A431) cell line. The observed effects may be additive or synergistic effects in inducing ROS generation and apoptosis, and reducing the viability of A431 cells.
癌症被认为是最具危害性的，因为它在全球范围内导致的死亡率很高。在这些癌症中，皮肤癌的影响最大，全球每三个癌症患者中就有一个受到影响。据报道，约有 200 万至 300 万例癌症是非黑色素瘤和黑色素瘤皮肤癌。尽管化疗药物作用于癌细胞，但会导致长期的病态，影响人们的生活质量，而且仅在癌症的早期阶段有效。因此，研究人员追求用传统医学的理念来有效治疗疾病，同时减少副作用。我们评估了对羟基肉桂酸和柚皮苷联合使用对 A431（表皮样癌细胞）发挥抗癌活性的效果。联合用药对 A431 细胞的 MTT 分析显示，最低的 IC 浓度为 41μg/ml，比标准药物咪喹莫特（IC 浓度为 52μg/ml）更有效。此外，还进行了流式细胞术分析，以确定联合用药抗癌作用的分子机制。结果表明，与对照组相比，该联合用药将 A431 细胞周期阻滞在 S 期，诱导细胞凋亡，早期和晚期凋亡细胞增多，进一步改变 A431 细胞中的活性氧（ROS）和线粒体膜电位。因此，结果表明对羟基肉桂酸和柚皮苷联合用药具有潜在的抗癌作用，可对抗皮肤癌（A431）细胞系。观察到的作用可能是通过增加 ROS 生成和诱导细胞凋亡，减少 A431 细胞活力而产生的相加或协同作用。
