Division of Uterine Vascular Biology, Guangzhou Institute of Pediatrics, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, China.
Methods Mol Biol. 2024;2728:183-194. doi: 10.1007/978-1-0716-3495-0_16.
During early pregnancy, fetal-derived extravillous trophoblast cells (EVT) from the placenta invade the maternal decidua and inner third of the uterus where they establish fetal tolerance and remodel the uterine spiral arteries, which ensures establishment of a successful pregnancy. Aberrant EVT invasion and spiral artery remodeling is associated with a number of pregnancy complications including miscarriage, preeclampsia, fetal growth restriction, and placenta accrete. During invasion of the maternal tissues, the EVT interact with a number of different cell types including the decidual leukocytes. EVT express HLA-C, HLA-G, HLA-E, and HLA-F and interact with uterine natural killer (uNK) cells through a series of different receptors. Epidemiological evidence suggests that different combinations of HLA-C and killer cell Ig-like receptor (KIR) haplotypes impact pregnancy success. Therefore, there is much interest in the functional consequence of interactions between EVT and uNK cells, and several different methodologies have been used to isolate these different cell types and their co-culture.
在妊娠早期，胎盘来源的胎儿绒毛外滋养层细胞（EVT）侵入母体蜕膜和子宫内层，在那里建立胎儿耐受性并重塑子宫螺旋动脉，从而确保成功妊娠。异常的 EVT 浸润和螺旋动脉重塑与许多妊娠并发症有关，包括流产、子痫前期、胎儿生长受限和胎盘粘连。在侵入母体组织的过程中，EVT 与包括蜕膜白细胞在内的许多不同类型的细胞相互作用。EVT 表达 HLA-C、HLA-G、HLA-E 和 HLA-F，并通过一系列不同的受体与子宫自然杀伤 (uNK) 细胞相互作用。流行病学证据表明，不同的 HLA-C 和杀伤细胞免疫球蛋白样受体 (KIR) 单倍型组合对妊娠成功有影响。因此，EVT 和 uNK 细胞之间相互作用的功能后果引起了广泛关注，已经使用了几种不同的方法来分离这些不同的细胞类型及其共培养。
