三维滋养细胞侵袭微流控平台用于毒理学筛选。
A Three-Dimensional Trophoblast Invasion Microfluidic Platform for Toxicological Screening.
机构信息
Department of Pathology, University of Illinois at Chicago, Chicago, IL, USA.
The Chicago Center for Health and Environment, University of Illinois at Chicago, Chicago, IL, USA.
出版信息
Methods Mol Biol. 2024;2728:223-234. doi: 10.1007/978-1-0716-3495-0_18.
Abstract
To improve our understanding of human placental function and placental cell responses to pregnancy stressors, the development of in vitro models that better recapitulate the in vivo placental microenvironment is needed. Here, we describe a three-dimensional (3D) silicone polymer polydimethylsiloxane (PDMS) microfluidic platform for modeling human trophoblast invasion recreating a placental heterocellular microenvironment. This platform allows the formation of a cellular barrier establishing a chemical gradient and real-time evaluation of trophoblast cell invasion and heterocellular cell-to-cell interactions.
摘要
为了增进我们对于人类胎盘功能和胎盘细胞对妊娠应激反应的理解，需要开发更好地模拟体内胎盘微环境的体外模型。在此，我们描述了一种用于模拟人类滋养层细胞侵袭以再现胎盘异细胞微环境的三维（3D）硅酮聚合物聚二甲基硅氧烷（PDMS）微流控平台。该平台允许形成建立化学梯度的细胞屏障，并实时评估滋养层细胞侵袭和异细胞细胞间相互作用。
相似文献
1
A Three-Dimensional Trophoblast Invasion Microfluidic Platform for Toxicological Screening.三维滋养细胞侵袭微流控平台用于毒理学筛选。
Methods Mol Biol. 2024;2728:223-234. doi: 10.1007/978-1-0716-3495-0_18.
2
A 3-dimensional microfluidic platform for modeling human extravillous trophoblast invasion and toxicological screening.一种用于模拟人绒毛外滋养层细胞侵袭和毒理学筛选的三维微流控平台。
Lab Chip. 2021 Feb 9;21(3):546-557. doi: 10.1039/d0lc01013h.
3
A microfluidics assay to study invasion of human placental trophoblast cells.一种用于研究人胎盘滋养层细胞侵袭的微流控分析方法。
J R Soc Interface. 2017 May;14(130). doi: 10.1098/rsif.2017.0131.
4
TGFβ signalling: a nexus between inflammation, placental health and preeclampsia throughout pregnancy.TGFβ 信号通路：贯穿整个孕期的炎症、胎盘健康与子痫前期之间的关联。
Hum Reprod Update. 2024 Jul 1;30(4):442-471. doi: 10.1093/humupd/dmae007.
5
Placenta-on-a-chip: a novel platform to study the biology of the human placenta.芯片上的胎盘：研究人类胎盘生物学的新型平台。
J Matern Fetal Neonatal Med. 2016;29(7):1046-54. doi: 10.3109/14767058.2015.1038518. Epub 2015 Jun 15.
6
Investigation of human trophoblast invasion in vitro.体外研究人滋养层细胞浸润。
Hum Reprod Update. 2020 Jun 18;26(4):501-513. doi: 10.1093/humupd/dmaa017.
7
Three-Dimensional In Vitro Human Placental Organoids from Mononuclear Villous Trophoblasts or Trophoblast Stem Cells to Understand Trophoblast Dysfunction in Fetal Growth Restriction.从单核绒毛滋养细胞或滋养干细胞中生成三维体外人胎盘类器官，以了解胎儿生长受限中的滋养细胞功能障碍。
Methods Mol Biol. 2024;2728:235-245. doi: 10.1007/978-1-0716-3495-0_19.
8
Trophoblast stem cells, trophoblast invasion, and organoids - advancements in gynecology.滋养层干细胞、滋养层侵袭和类器官——妇科的进展。
Ceska Gynekol. 2024;89(2):151-155. doi: 10.48095/cccg2024151.
9
A Microphysiological Model to Mimic the Placental Remodeling during Early Stage of Pregnancy under Hypoxia-Induced Trophoblast Invasion.一种用于模拟缺氧诱导滋养层细胞侵袭下妊娠早期胎盘重塑的微生理模型。
Biomimetics (Basel). 2024 May 12;9(5):289. doi: 10.3390/biomimetics9050289.
10
Something old, something new: digital quantification of uterine vascular remodelling and trophoblast plugging in historical collections provides new insight into adaptation of the utero-placental circulation.旧中有新：对历史标本中子宫血管重塑和绒毛血栓的数字化定量分析为子宫胎盘循环的适应提供了新的见解。
Hum Reprod. 2021 Feb 18;36(3):571-586. doi: 10.1093/humrep/deaa303.
本文引用的文献
1
A 3-dimensional microfluidic platform for modeling human extravillous trophoblast invasion and toxicological screening.一种用于模拟人绒毛外滋养层细胞侵袭和毒理学筛选的三维微流控平台。
Lab Chip. 2021 Feb 9;21(3):546-557. doi: 10.1039/d0lc01013h.
2
A gelatin hydrogel to study endometrial angiogenesis and trophoblast invasion.一种用于研究子宫内膜血管生成和滋养层细胞侵袭的明胶水凝胶。
Interface Focus. 2019 Oct 6;9(5):20190016. doi: 10.1098/rsfs.2019.0016. Epub 2019 Aug 16.
3
Transcriptomic and functional analyses of 3D placental extravillous trophoblast spheroids.三维胎盘绒毛外滋养层球体的转录组和功能分析。
Sci Rep. 2019 Aug 30;9(1):12607. doi: 10.1038/s41598-019-48816-8.
4
A microfluidics assay to study invasion of human placental trophoblast cells.一种用于研究人胎盘滋养层细胞侵袭的微流控分析方法。
J R Soc Interface. 2017 May;14(130). doi: 10.1098/rsif.2017.0131.
5
Defective trophoblast invasion underlies fetal growth restriction and preeclampsia-like symptoms in the stroke-prone spontaneously hypertensive rat.胚胎滋养层细胞入侵缺陷是易卒中型自发性高血压大鼠胎儿生长受限和子痫前期样症状的基础。
Mol Hum Reprod. 2017 Jul 1;23(7):509-519. doi: 10.1093/molehr/gax024.
6
Only humans have human placentas: molecular differences between mice and humans.只有人类拥有人类胎盘：小鼠与人类之间的分子差异。
J Reprod Immunol. 2015 Apr;108:65-71. doi: 10.1016/j.jri.2015.03.001. Epub 2015 Mar 12.
7
In vitro cell migration and invasion assays.体外细胞迁移和侵袭实验。
Mutat Res. 2013 Jan-Mar;752(1):10-24. doi: 10.1016/j.mrrev.2012.08.001. Epub 2012 Aug 23.
8
In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro.体外划痕试验：一种用于体外细胞迁移分析的简便且经济的方法。
Nat Protoc. 2007;2(2):329-33. doi: 10.1038/nprot.2007.30.
9
Defective endovascular trophoblast invasion in primary antiphospholipid antibody syndrome-associated early pregnancy failure.原发性抗磷脂抗体综合征相关早期妊娠失败中血管内滋养层细胞侵袭缺陷。
Hum Reprod. 2002 Apr;17(4):1067-71. doi: 10.1093/humrep/17.4.1067.
10
Human cytotrophoblast differentiation/invasion is abnormal in pre-eclampsia.子痫前期患者的人细胞滋养层细胞分化/侵袭异常。
Am J Pathol. 1997 Dec;151(6):1809-18.
Zotero 插件