Department of Pathology, University of Illinois at Chicago, Chicago, IL, USA.
The Chicago Center for Health and Environment, University of Illinois at Chicago, Chicago, IL, USA.
Methods Mol Biol. 2024;2728:223-234. doi: 10.1007/978-1-0716-3495-0_18.
To improve our understanding of human placental function and placental cell responses to pregnancy stressors, the development of in vitro models that better recapitulate the in vivo placental microenvironment is needed. Here, we describe a three-dimensional (3D) silicone polymer polydimethylsiloxane (PDMS) microfluidic platform for modeling human trophoblast invasion recreating a placental heterocellular microenvironment. This platform allows the formation of a cellular barrier establishing a chemical gradient and real-time evaluation of trophoblast cell invasion and heterocellular cell-to-cell interactions.
为了增进我们对于人类胎盘功能和胎盘细胞对妊娠应激反应的理解，需要开发更好地模拟体内胎盘微环境的体外模型。在此，我们描述了一种用于模拟人类滋养层细胞侵袭以再现胎盘异细胞微环境的三维（3D）硅酮聚合物聚二甲基硅氧烷（PDMS）微流控平台。该平台允许形成建立化学梯度的细胞屏障，并实时评估滋养层细胞侵袭和异细胞细胞间相互作用。
