Heterologous expression of the cryptic gene cluster and structural revision of maduralactomycin A.

After conducting an analysis of the cryptic cluster region and performing transcriptomic studies, an integrative BAC Vector containing the gene sequence was constructed. The heterologous expression of the cluster in J1074 resulted in the production of the angucyclic product, seongomycin, which allowed for the assesment of its antibacterial, antiproliferative, and antiviral activities. Heterologous production was further confirmed by targeted knock-out experiments involving key regulators of the biosynthetic pathways. We were further able to revise the core structure of maduralactomycin A, using a computational approach.