Zaburannyi Nestor, Rabyk Mariia, Ostash Bohdan, Fedorenko Victor, Luzhetskyy Andriy
Helmholtz-Institute for Pharmaceutical Research Saarland, Saarland University Campus, Building C2,3, 66123 Saarbrücken, Germany.
BMC Genomics. 2014 Feb 5;15:97. doi: 10.1186/1471-2164-15-97.
The Streptomyces albus J1074 strain is one of the most widely used chassis for the heterologous production of bioactive natural products. The fast growth and an efficient genetic system make this strain an attractive model for expressing cryptic biosynthetic pathways to aid drug discovery.
To improve its capabilities for the heterologous expression of biosynthetic gene clusters, the complete genomic sequence of S. albus J1074 was obtained. With a size of 6,841,649 bp, coding for 5,832 genes, its genome is the smallest within the genus streptomycetes. Genome analysis revealed a strong tendency to reduce the number of genetic duplicates. The whole transcriptomes were sequenced at different time points to identify the early metabolic switch from the exponential to the stationary phase in S. albus J1074.
S. albus J1074 carries the smallest genome among the completely sequenced species of the genus Streptomyces. The detailed genome and transcriptome analysis discloses its capability to serve as a premium host for the heterologous production of natural products. Moreover, the genome revealed 22 additional putative secondary metabolite gene clusters that reinforce the strain's potential for natural product synthesis.
白色链霉菌J1074菌株是用于生物活性天然产物异源生产的最广泛使用的底盘之一。其快速生长和高效的遗传系统使其成为表达隐秘生物合成途径以辅助药物发现的有吸引力的模型。
为了提高其生物合成基因簇的异源表达能力，获得了白色链霉菌J1074的完整基因组序列。其基因组大小为6,841,649 bp，编码5,832个基因，是链霉菌属中最小的。基因组分析显示出减少基因重复数量的强烈趋势。在不同时间点对整个转录组进行测序，以确定白色链霉菌J1074从指数期到稳定期的早期代谢转变。
白色链霉菌J1074在链霉菌属已完全测序的物种中拥有最小的基因组。详细的基因组和转录组分析揭示了其作为天然产物异源生产优质宿主的能力。此外，基因组还揭示了另外22个推定的次生代谢物基因簇，增强了该菌株天然产物合成的潜力。
