Ross F M, Stockdill G
Cancer Genet Cytogenet. 1987 Mar;25(1):109-21. doi: 10.1016/0165-4608(87)90166-x.
Cells from 52 patients with chronic lymphocytic leukemia and two patients with prolymphocytic leukemia were stimulated in whole blood or bone marrow culture with the polyclonal B-cell mitogen tetradecanoyl phorbol acetate (TPA). Stimulation was successful in 50 of the 54 cases. Comparison with Epstein-Barr virus and pokeweed mitogen showed them to be much less successful mitogens under the conditions used. Twenty-one patients (39%) were found to have clonal abnormalities that were probably disease-related. The most common abnormality was trisomy 12 (five patients). Four patients had a deletion of the long arm of chromosome #11 and one patient had a t(11;14). Five patients had abnormalities of the long arm of chromosome #13, with four probably having breaks in band q14. Two patients were seen with trisomy 3 as the only abnormality. Overall, the results were similar to those of other groups, which shows that considerable karyotypic information can be obtained from the use of a single mitogen on a large series of chronic lymphocytic leukemia patients.
