Geng YanXia, Fei ShuYe, Pei YingHao, Chen QiuHua, Wang Jian, Jiang Hua
Department of Intensive Care Unit, The Affiliated Hospitalof Nanjing University of Chinese Medicine, Jiangsu Province Hospital of Chinese Medicine, 155 Han Zhong Road, Nanjing, Jiangsu, China.
Nanjing University of Chinese Medicine, 282 Han Zhong Road, Nanjing, Jiangsu, China.
J Tradit Complement Med. 2023 Apr 25;13(6):531-537. doi: 10.1016/j.jtcme.2023.04.001. eCollection 2023 Nov.
Sepsis is now a global medical burden with high morbility and mortality. The focus of this study was to evaluate the effects of Ziqi Dihuang (ZQDH) decoction on inflammatory and thrombosis-related parameters in septic rats.
A rat model of sepsis was established by cecal ligation and puncture (CLP). Male Sprague-Dawley rats were randomly divided into Sham group, CLP group, ZQDH-1ow group (0.735 g/kg) and ZQDH-high group (1.47 g/kg). Rats in ZQDH groups were given ZQDH decoction by gavage for 7 days before CLP. White blood cells (WBC), inflammatory cell infiltration of liver, kidney and lung, as well as serum levels of tumor necrosis factor (TNF-α), interleukin-6 (IL-6) and reactive oxygen species (ROS) were used to assess systemic inflammatory response. Coagulation and fibrinolytic indexes included platelet count, coagulation function, fibrin deposition, and levels of tissue plasminogen activator (tPA) and plasminogen activator inhibitor-1 (PAI-1) in serum, liver, kidney and lung.
LPS rats showed significant changes in inflammatory and thrombosis-related parameters such as increased WBC and inflammatory factors, decreased platelet counts, and increased tPA and PAI-1 concentrations in serum and organs. ZQDH decoction pretreatment can significantly inhibit the infiltration of inflammatory cells in the lung, and inhibit the production of TNF-α, IL-6 and ROS in a dose-dependent manner. ZQDH decoction also ameliorated thrombocytopenia, renal fibrin deposition, and tPA and PAI-1 levels in serum and organs.
These results suggest that ZQDH decoction can dose-dependently relieve systemic inflammatory injury and regulate fibrinolysis system in septic rats, which may be mediated by PAI-1.
脓毒症目前是一个全球性的医学负担，具有高发病率和死亡率。本研究的重点是评估紫芪地黄汤对脓毒症大鼠炎症和血栓形成相关参数的影响。
通过盲肠结扎和穿刺（CLP）建立脓毒症大鼠模型。雄性Sprague-Dawley大鼠随机分为假手术组、CLP组、紫芪地黄汤低剂量组（0.735 g/kg）和紫芪地黄汤高剂量组（1.47 g/kg）。紫芪地黄汤组大鼠在CLP前7天通过灌胃给予紫芪地黄汤。采用白细胞（WBC）、肝、肾和肺的炎症细胞浸润以及血清肿瘤坏死因子（TNF-α）、白细胞介素-6（IL-6）和活性氧（ROS）水平评估全身炎症反应。凝血和纤溶指标包括血小板计数、凝血功能、纤维蛋白沉积以及血清、肝、肾和肺中组织型纤溶酶原激活剂（tPA）和纤溶酶原激活剂抑制剂-1（PAI-1）的水平。
脂多糖（LPS）大鼠在炎症和血栓形成相关参数方面表现出显著变化，如白细胞和炎症因子增加、血小板计数减少以及血清和器官中tPA和PAI-1浓度增加。紫芪地黄汤预处理可显著抑制肺中炎症细胞浸润，并以剂量依赖方式抑制TNF-α、IL-6和ROS的产生。紫芪地黄汤还改善了血小板减少症、肾纤维蛋白沉积以及血清和器官中tPA和PAI-1水平。
这些结果表明，紫芪地黄汤可剂量依赖性地减轻脓毒症大鼠的全身炎症损伤并调节纤溶系统，这可能由PAI-1介导。
