Mutations of RAS genes identified in acute myeloid leukemia affect glycerophospholipid metabolism pathway.

BACKGROUND

Acute myeloid leukemia (AML) is a malignant disease originating from myeloid hematopoietic stem cells. Recent studies have shown that certain gene mutations promote tumor cell survival and affect the prognosis of patients by affecting metabolic mechanisms in tumor cells. gene mutations are prevalent in AML, and the signaling pathway is closely related to many metabolic pathways. However, the effects of different gene mutations on AML cell metabolism are unclear.

OBJECTIVES

The main purpose of this study was to explore the effect of gene mutation on the metabolic pathway of tumor cells.

METHODS

In this study, we first used a retrovirus carrying a mutant gene to prepare Ba/F3 cell lines with gene mutations, and then compared full-transcriptome data of Ba/F3 cells before and after gene mutation and found that differentially expressed genes after NRAS and KRAS mutation.

RESULTS

We found a total of 1899 differentially expressed genes after NRAS and KRAS mutation. 1089 of these genes were involved in metabolic processes, of which 167 genes were enriched in metabolism-related pathways. In metabolism-related pathways, differential genes were associated with the lipid metabolism pathway. Moreover, by comparing groups, we found that the expression of the and genes in the glycerophospholipid metabolism pathway was significantly upregulated.

CONCLUSION

In conclusion, our study revealed that gene mutation is closely related to the glycerophospholipid metabolism pathway in Ba/F3 cells, which may contribute to new precision therapy strategies and the development and application of new therapeutic drugs for AML.