Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Toruń, Wileńska 4 Str., Toruń 87-100, Poland.
Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Toruń, Wileńska 4 Str., Toruń 87-100, Poland.
Diagn Microbiol Infect Dis. 2024 Feb;108(2):116150. doi: 10.1016/j.diagmicrobio.2023.116150. Epub 2023 Nov 25.
The emergence of Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI TOF MS) technology has expanded the capabilities for identifying microorganisms in clinical labs, replacing traditional biochemical testing with a proteomic approach. In the present study, we compared results between the two commercial MALDI TOF MS systems, Bruker Microflex LT Biotyper and Zybio EXS2600 Ex-Accuspec, for the identification of 1979 urinary isolates by direct extraction method. Current study found that both systems identified a high percentage of isolates to at least the genus level - Bruker 95.6 % of isolates, Zybio 92.4 %. In the case of 89.5 % of all analyzed spectra, the identification results were consistent between the used devices. The highest score values and the highest percentage of spectra identified to species were obtained for gram-negative bacteria. The results show that both systems are equally good choices in terms of analytical performance for routine microbiological diagnostic procedures.
基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）技术的出现扩展了临床实验室中鉴定微生物的能力，用蛋白质组学方法取代了传统的生化检测。在本研究中，我们比较了两种商用 MALDI-TOF MS 系统（布鲁克 Microflex LT Biotyper 和 Zybio EXS2600 Ex-Accuspec）对直接提取法鉴定 1979 株尿分离株的结果。目前的研究发现，这两种系统都能将很大比例的分离株鉴定到至少属的水平 - 布鲁克为 95.6%，Zybio 为 92.4%。在所有分析谱的 89.5%的情况下，两种设备的鉴定结果是一致的。革兰氏阴性菌的得分值最高，鉴定到种的谱的比例也最高。结果表明，这两种系统在常规微生物诊断程序的分析性能方面都是同等的选择。
