Schwendenmann Nadja, Burger Sophie, Steiner Leonie, Seufert Lars, Kohl Matthias, Mack Matthias, Egert Markus
Faculty of Health, Medical and Life Sciences, Microbiology and Hygiene Group, Institute of Precision Medicine, Furtwangen University of Applied Sciences, Villingen-Schwenningen, Germany.
Institute of Technical Microbiology, Mannheim University of Applied Sciences, Mannheim, Germany.
Folia Microbiol (Praha). 2025 Sep 17. doi: 10.1007/s12223-025-01317-w.
The antivitamin roseoflavin (RoF) is a structural analogue and competitive inhibitor of riboflavin (RF, vitamin B). Stimulated by a recent report that cariogenic Streptococcus mutans is prototrophic for RF, we conducted pilot investigations on the effect of RF and RoF on the growth of selected microbial isolates from human saliva to lay a first foundation whether oral application of RoF might be suitable to modify the human oral microbiota for the sake of human health. Microbial isolates from saliva samples of 41 healthy volunteers were obtained and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Thirty different microbial isolates were investigated regarding growth promotion by RF and susceptibility to RoF using growth experiments. Isolates were grown in RF-free medium without supplements or supplemented with 100 µmol/L RF, 100 µmol/L RoF, or a combination of both, respectively. Absorbance (A) was measured before and after 24 h of incubation using a spectrophotometer. Log2-transformed absorbance data were analyzed using analysis of covariance (ANCOVA). Mainly the growth of gram-positive bacteria was affected by RF and RoF. Growth of 5 gram-positive species from the genera Enterococcus, Staphylococcus, and Streptococcus was significantly reduced upon addition of RoF when compared to growth without any additives. Simultaneous administration of RF and RoF led to significantly reduced growth of at least 3 gram-positive species from the genera Bacillus and Staphylococcus when compared to growth with RF alone. Growth of cariogenic S. mutans was significantly enhanced by RF, and this effect was at least partly, but not significantly, reversed by supplementing the growth media with RoF. In agreement with previous investigations, our data suggests that gram-positive bacteria in the human oral cavity might be more affected by RoF than gram-negatives. Our future research aims at characterizing the inhibited bacteria, the underlying molecular mechanism(s), and the effect of RoF on the composition of the complex microbial community in the human oral cavity in more detail.
抗维生素玫瑰黄素（RoF）是核黄素（RF，维生素B）的结构类似物和竞争性抑制剂。受近期一份关于致龋变形链球菌对RF为原养型的报告的启发，我们对RF和RoF对从人类唾液中分离出的特定微生物菌株生长的影响进行了初步研究，以初步确定口服RoF是否可能适合为了人类健康而改变人类口腔微生物群。通过基质辅助激光解吸/电离飞行时间质谱法从41名健康志愿者的唾液样本中获取并鉴定微生物菌株。使用生长实验研究了30种不同的微生物菌株对RF促进生长的作用以及对RoF的敏感性。菌株分别在无补充剂的无RF培养基中、添加100 μmol/L RF、100 μmol/L RoF或两者组合的培养基中生长。使用分光光度计在孵育24小时前后测量吸光度（A）。使用协方差分析（ANCOVA）分析对数2转换后的吸光度数据。主要是革兰氏阳性菌的生长受到RF和RoF的影响。与无任何添加剂时的生长相比，添加RoF后，肠球菌属、葡萄球菌属和链球菌属的5种革兰氏阳性菌的生长显著降低。与仅用RF培养相比，同时给予RF和RoF导致芽孢杆菌属和葡萄球菌属中至少3种革兰氏阳性菌的生长显著降低。致龋变形链球菌的生长因RF而显著增强，并且通过在生长培养基中添加RoF，这种作用至少部分但不显著地被逆转。与先前的研究一致，我们的数据表明，人类口腔中的革兰氏阳性菌可能比革兰氏阴性菌更容易受到RoF的影响。我们未来的研究旨在更详细地表征受抑制的细菌、潜在的分子机制以及RoF对人类口腔复杂微生物群落组成的影响。
