Flexible Electronics (IFE, Future Technologies), Xiamen University, Xiamen, 361005, China.
Future Display Institute in Xiamen, Xiamen, 361005, China.
Chemistry. 2024 Feb 26;30(12):e202303208. doi: 10.1002/chem.202303208. Epub 2024 Jan 8.
Fluorophores are considered powerful tools for not only enabling the visualization of cell structures, substructures, and biological processes, but also making for the quantitative and qualitative measurement of various analytes in living systems. However, most fluorophores do not meet the diverse requirements for biological applications in terms of their photophysical and biological properties. Hybridization is an important strategy in molecular engineering that provides fluorophores with complementarity and multifunctionality. This review summarizes the basic strategies of hybridization with four classes of fluorophores, including xanthene, cyanine, coumarin, and BODIPY with a focus on their structure-property relationship (SPR) and biological applications. This review aims to provide rational hybrid ideas for expanding the reservoir of knowledge regarding fluorophores and promoting the development of newly produced fluorophores for applications in the field of life sciences.
荧光团不仅被认为是可视化细胞结构、亚结构和生物过程的有力工具，而且还可以定量和定性测量活系统中的各种分析物。然而，大多数荧光团在光物理和生物学性质方面不能满足生物应用的多样化要求。杂交是分子工程中的一种重要策略，它为荧光团提供了互补性和多功能性。本文综述了四种类型的荧光团（呫吨、花菁、香豆素和 BODIPY）的杂交基本策略，重点介绍了它们的结构-性质关系（SPR）和生物应用。本文旨在为扩展荧光团的知识库和促进新型荧光团在生命科学领域的应用提供合理的杂交思路。
