基于聚乙烯醇衍生碳量子点的荧光“开-关”探针用于牛奶和鸡蛋样品中莫西沙星的检测。

Polyvinyl alcohol-derived-carbon quantum dots based fluorometric "On-Off" probe for moxifloxacin detection in milk and egg samples.

机构信息

Nano-bio Laboratory, Special Center for Nanoscience, Jawaharlal Nehru University, New Delhi 110067, India.

Nano-bio Laboratory, Special Center for Nanoscience, Jawaharlal Nehru University, New Delhi 110067, India; Department of Biotechnology, School of Life Sciences, Mahatma Gandhi Central University, Motihari, Bihar 845401, India.

出版信息

Food Chem. 2024 May 1;439:138038. doi: 10.1016/j.foodchem.2023.138038. Epub 2023 Nov 23.

DOI:10.1016/j.foodchem.2023.138038

PMID:38041884

Abstract

Unconditional use of antibiotics triggered the process of bacterial resistance and causes major health problems. Nowadays, antibiotics majorly used in animals not only for infection treatment but also as mass promotor. The excess amount of antibiotics residue in animal derived foods which accelerate antibiotic resistance (ABR). So, here, a simple and quick carbon quantum dots(CQDs) based fluorometric "On-Off" probe was developed for detection of moxifloxacin (MOXI) in milk and egg samples. The CQDs emits blue emission and are uniformly distributed with average particle size 5.9 ± 0.22 nm. With MOXI, fluorescence intensity of CQDs at 372 nm decreased due to inner filter effect (IFE) and a new peak appeared at 508 nm correspondence to MOXI. The probe shows linear response with MOXI concentration varies as 0.025 µM - 15.0 µM with lower detection limit (LOD) of 6.34 nM. The real sample applicability test proved that the sensors have excellent efficacy for food applications.

摘要

抗生素的无条件使用引发了细菌耐药性的产生，并导致了重大的健康问题。如今，抗生素在动物中的主要用途不仅是治疗感染，还用作大量促进剂。动物源性食品中抗生素残留量过多，加速了抗生素耐药性（ABR）的产生。因此，在这里，我们开发了一种简单、快速的基于碳量子点（CQDs）的荧光“开-关”探针，用于检测牛奶和鸡蛋样品中的莫西沙星（MOXI）。CQDs 发出蓝色发射光，且分布均匀，平均粒径为 5.9±0.22nm。与 MOXI 结合后，由于内滤效应（IFE），CQDs 在 372nm 处的荧光强度降低，同时在 508nm 处出现与 MOXI 相对应的新峰。该探针对 MOXI 的浓度表现出线性响应，浓度范围为 0.025µM-15.0µM，检测限（LOD）为 6.34nM。实际样品应用测试证明，该传感器在食品应用中具有优异的效果。