Derayea Sayed M, Hammad Mohamed A, Omar Mahmoud A, Hassan Yasser F
Department of Analytical Chemistry, Faculty of Pharmacy, Minia University, Minia, 61519, Egypt.
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Minia National University, New Minia, Egypt.
BMC Chem. 2025 Jul 15;19(1):212. doi: 10.1186/s13065-025-01580-5.
This work deals with devising a quick and easy method for moxifloxacin assay using a sensitive spectrofluorimetric technique. The method benefited from the interaction of the aforementioned drug with the aluminum cation which form a stable chelate with a high fluorescence characteristic. Further improvement in the fluorescence was achieved upon the addition of sodium lauryl sulfate. The experimental parameters of the reaction were carefully investigated. The fluorescence intensity was measured at a wavelength of 478 nm after excitation at 365 nm. A linear relationship between the intensity of fluorescence and moxifloxacin concentration was in the range of 10-200 ng mL. The developed method was highly sensitive since the lower limit of detection was 2.2 ng mL. Application of the suggested spectrofluorimetric method included the analysis of commercial tablet dosage forms containing the cited drug. In addition, human plasma samples spiked with the drug were analyzed, and good recovery was obtained. Moreover, the environmental friendliness of the developed procedure was confirmed through employing AGREE and GAPI approaches.
这项工作致力于设计一种使用灵敏的荧光光谱技术快速简便地测定莫西沙星的方法。该方法利用了上述药物与铝阳离子的相互作用，铝阳离子与之形成具有高荧光特性的稳定螯合物。加入十二烷基硫酸钠后，荧光进一步增强。对反应的实验参数进行了仔细研究。在365nm激发后，于478nm波长处测量荧光强度。荧光强度与莫西沙星浓度在10 - 200 ng/mL范围内呈线性关系。所开发的方法高度灵敏，因为检测下限为2.2 ng/mL。所建议的荧光光谱法的应用包括对含有该药物的市售片剂剂型进行分析。此外，对加有该药物的人血浆样本进行了分析，并获得了良好的回收率。此外，通过采用AGREE和GAPI方法证实了所开发方法的环境友好性。
