Department of Neck Surgery, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China
Balkan Med J. 2024 Jan 3;41(1):23-29. doi: 10.4274/balkanmedj.galenos.2023.2023-7-123. Epub 2023 Dec 4.
Thyroid cancer (TC), the most prevalent endocrine malignancy, has been subjected to various treatment methods. However, the efficacy of asiaticoside (AC) for treating TC remains uncertain.
To explore the impact of AC on TC and determine its potential mechanisms of action.
In vitro and in vivo cell line study.
We evaluated the effects of AC on human TC cell lines, namely TPC-1 and FTC-133. Both in vitro and in vivo experimental validations were conducted.
AC significantly diminished the viability and proliferation of TC cells based on the CCK-8 assay and Edu staining findings. Migration and invasion assays revealed that AC effectively curtailed the migration and invasiveness of TC cells. The tube formation assay demonstrated that AC substantially impeded TC cell-induced angiogenesis. Western blot assay revealed that AC significantly reduced the expression levels of TRAF6, HIF-1α, and VEGFA, indicating that AC could potentially exert its anticancer effect by inhibiting the TRAF6/HIF1α pathway. Our in vivo experiments, which involved administering AC to BALB/c nude mice injected with TPC-1 cells, demonstrated significant inhibition of tumor growth and reduction in the expression of Ki-67, TRAF6, HIF-1α, and VEGFA.
Our study highlights the significant inhibitory effect of AC on TC, offering fresh insights and potential drug candidates for TC treatment.
甲状腺癌（TC）是最常见的内分泌恶性肿瘤，已经采用了多种治疗方法。然而，积雪草苷（AC）治疗 TC 的疗效仍不确定。
探索 AC 对 TC 的影响，并确定其潜在的作用机制。
体外和体内细胞系研究。
我们评估了 AC 对人 TC 细胞系 TPC-1 和 FTC-133 的影响。进行了体外和体内实验验证。
CCK-8 检测和 Edu 染色结果显示，AC 显著降低 TC 细胞的活力和增殖。迁移和侵袭实验表明，AC 有效抑制 TC 细胞的迁移和侵袭能力。管形成实验表明，AC 可显著抑制 TC 细胞诱导的血管生成。Western blot 实验表明，AC 显著降低了 TRAF6、HIF-1α 和 VEGFA 的表达水平，提示 AC 可能通过抑制 TRAF6/HIF1α 通路发挥其抗癌作用。我们在体内实验中，给注射 TPC-1 细胞的 BALB/c 裸鼠给予 AC，结果显示肿瘤生长明显受到抑制，Ki-67、TRAF6、HIF-1α 和 VEGFA 的表达减少。
本研究强调了 AC 对 TC 的显著抑制作用，为 TC 治疗提供了新的见解和潜在的药物候选物。
