Montes-Robledo Alfredo, Buelvas-Montes Yaleyvis, Baldiris-Avila Rosa
Department of Exact and Natural Sciences, School of Biology, Universidad de Cartagena, Cartagena, Colombia.
Department of Basic Health, School of Medicine, Universidad del Sinú Cartagena, Cartagena, Colombia.
Iran J Microbiol. 2023 Aug;15(4):503-512. doi: 10.18502/ijm.v15i4.13504.
Extraintestinal pathogenic (ExPEC) is a recently recognized and highly diverse pathotype of Its significance as a pathogen has increased due to the emergence of hypervirulent and multidrug-resistant (MDR) strains. The aim of this study was to characterize ExPEC isolates from humans based on their phylogenetic group, virulence factor profile, and antimicrobial susceptibility.
The isolates were collected from patients with extraintestinal infections caused by , including urinary tract infections, bacteremia, and surgical site infections. The phylogenetic groups were determined using multiplex PCR. Additionally, the isolates were evaluated for their biofilm-forming abilities, susceptibility to antimicrobial agents, and presence of virulence genes.
In this study, the isolates were classified into four phylogenetic groups: A (48.3%), B2 (25.8%), D (19.35%), and B1 (6.45%). All isolates exhibited at least one of the ten analyzed virulence factors. However, there was no direct evidence linking a specific phylogenetic group to a particular virulence factor. Nevertheless, the presence of the , and virulence genes was correlated with the production of strong biofilms, multidrug resistance (MDR), and the production of alpha hemolysin.
This study provides a description of the phylogenetic groups in ExPEC and their potential association with virulence factor profiles and antimicrobial susceptibility.
肠外致病性大肠杆菌（ExPEC）是一种最近才被认识且具有高度多样性的致病类型。由于高毒力和多重耐药（MDR）菌株的出现，其作为病原菌的重要性日益增加。本研究的目的是根据系统发育群、毒力因子谱和抗菌药物敏感性对从人类分离出的ExPEC进行特征描述。
分离株取自由大肠杆菌引起的肠外感染患者，包括尿路感染、菌血症和手术部位感染。使用多重PCR确定大肠杆菌的系统发育群。此外，对分离株的生物膜形成能力、对抗菌药物的敏感性以及毒力基因的存在情况进行评估。
在本研究中，分离株被分为四个系统发育群：A群（48.3%）、B2群（25.8%）、D群（19.35%）和B1群（6.45%）。所有分离株均表现出所分析的十种毒力因子中的至少一种。然而，没有直接证据表明特定的系统发育群与特定的毒力因子相关。尽管如此， fimH、papC和hlyA毒力基因的存在与强生物膜的产生、多重耐药（MDR）以及α溶血素的产生相关。
本研究对ExPEC中的系统发育群及其与毒力因子谱和抗菌药物敏感性的潜在关联进行了描述。
