Prunieras M
Arch Dermatol Res. 1979 Mar 31;264(2):243-7. doi: 10.1007/BF00431135.
Among the many skin culture systems, three have been selected in this short review because of their specific potentials in dermatological research. H. Green cultures newborn human forsekin keratinocytes on a mouse 3T3 feeder layer. Keratinocytes grow and keratinize. The feeder cells release factor(a) which allows serial propagation of keratinocytes to be achieved. The cell yield is further increased by adding epidermal grohth factor. This system has already proved to be a potent tool for the study of keratinization at the molecular level. A. Freeman has described a system in which explants of adult human skin are cultured on the dermal aspect of dead split-thickness pig skin. Keratinocytes can be passaged several times. Their differentiation is remarkable: it includes the production of keratohyaline, membrane coating granules, pemphigus as well as pemphigoid antigens. This system is interesting in the study of epidermal morphogenesis and may be applicable to the treatment of burns. The culture of epidermal cells from adult guinea pig ear in comparison with that of dermal fibroblasts is being used to study the specificity of action of pharmacological compounds on growth and keratinization of epidermal cells. Furthermore, the isolation (and culture) of pure populations of basal cells appears as a promising approach to the study of the mechanisms which moderate epidermal cell proliferation.
在众多皮肤培养系统中，本次简短综述挑选了三种，因其在皮肤病学研究中具有特定潜力。H. 格林在小鼠3T3饲养层上培养新生儿人类包皮角质形成细胞。角质形成细胞生长并角质化。饲养细胞释放因子(a)，从而实现角质形成细胞的连续传代培养。添加表皮生长因子可进一步提高细胞产量。该系统已被证明是在分子水平研究角质化的有力工具。A. 弗里曼描述了一种系统，即将成人皮肤外植体培养在死亡的断层猪皮的真皮面上。角质形成细胞可以传代几次。它们的分化很显著：包括透明角质颗粒、膜被颗粒、天疱疮以及类天疱疮抗原的产生。该系统在表皮形态发生研究中很有意义，可能适用于烧伤治疗。将成年豚鼠耳部的表皮细胞培养物与真皮成纤维细胞的培养物进行比较，用于研究药理化合物对表皮细胞生长和角质化的作用特异性。此外，分离（并培养）纯基底细胞群体似乎是研究调节表皮细胞增殖机制的一种有前景的方法。
