Laboratório de Referência Nacional em Hidatidose - LRNH, Laboratório de Parasitologia Integrativa e Paleoparasitologia - LPIP, Instituto Oswaldo Cruz - IOC, Fundação Oswaldo Cruz - Fiocruz, Rio de Janeiro, RJ, Brasil.
Rev Bras Parasitol Vet. 2023 Dec 4;32(4):e012723. doi: 10.1590/S1984-29612023078. eCollection 2023.
The coproparasitological examination of dogs (n=278) from two Brazilian biomes (Amazon [AZ] and Atlantic Forest [AF]) by centrifugal flotation demonstrated positivity values of 54.2% (AF) and 48.5% (AZ). The most prevalent parasites in AF were hookworms (81.0% - 47/58), Toxocara sp. (17.3% - 10/58) and Trichuris vulpis (12.1% - 7/58); while in AZ they were hookworms (86.7% - 72/83), Toxocara sp. (18.1% - 15/83), Dipylidium caninum (13.3% - 11/83) and T. vulpis (10.8% - 9/83). PCR was performed using the partial mitochondrial genes cytochrome c oxidase subunit 1 (pcox1) and NADH dehydrogenase 1 (pnad1) in 25 fecal samples positive for Toxocara sp. eggs and found one sample positive for pcox1 and six positives for pnad1. The sequencing of these samples was unsuccessful due to the difficulties inherent in copro-PCR+sequencing. The sequencing of 14 samples of T. canis adult helminths retrieved 11 sequences of 414 bp for pcox1 and nine sequences of 358 bp for pnad1. The phylogenetic trees of these sequences confirmed the species T. canis. Intraspecific genetic variation was only observed for pnad1. This is the second study involving molecular analysis of T. canis in dogs from Brazil and adds new information through the use of pnad1.
对来自巴西两个生物群落（亚马逊[AZ]和大西洋森林[AF]）的 278 只狗进行的共生寄生虫学检查通过离心漂浮法显示出阳性率分别为 54.2%（AF）和 48.5%（AZ）。在 AF 中最常见的寄生虫是钩虫（81.0% - 47/58）、旋毛形线虫（17.3% - 10/58）和犬复孔绦虫（12.1% - 7/58）；而在 AZ 中，它们是钩虫（86.7% - 72/83）、旋毛形线虫（18.1% - 15/83）、双腔吸虫（13.3% - 11/83）和犬复孔绦虫（10.8% - 9/83）。使用粪便样本中的部分线粒体基因细胞色素 c 氧化酶亚基 1（pcox1）和 NADH 脱氢酶 1（pnad1）对 25 份阳性的旋毛形线虫卵进行 PCR 检测，发现 1 份样本对 pcox1 呈阳性，6 份样本对 pnad1 呈阳性。由于 copro-PCR+测序固有的困难，这些样本的测序不成功。对 14 条犬复孔绦虫成虫蠕虫样本进行测序，得到了 11 个 414 bp 的 pcox1 序列和 9 个 358 bp 的 pnad1 序列。这些序列的系统发育树证实了犬复孔绦虫的物种地位。仅在 pnad1 中观察到种内遗传变异。这是第二项涉及巴西犬旋毛形线虫分子分析的研究，通过使用 pnad1 提供了新的信息。
