Mikaeili F, Mirhendi H, Mohebali M, Hosseini M, Sharbatkhori M, Zarei Z, Kia E B
Department of Medical Parasitology and Mycology,School of Public Health, Tehran University of Medical Sciences,Tehran,Iran.
Department of Medical Parasitology and Mycology,School of Public Health, National Institute of Health Research, Tehran University of Medical Sciences,Tehran,Iran.
J Helminthol. 2015 Jul;89(4):496-501. doi: 10.1017/S0022149X14000133. Epub 2014 Apr 9.
The study was conducted to determine the sequence variation in two mitochondrial genes, namely cytochrome c oxidase 1 (pcox1) and NADH dehydrogenase 1 (pnad1) within and among isolates of Toxocara cati, Toxocara canis and Toxascaris leonina. Genomic DNA was extracted from 32 isolates of T. cati, 9 isolates of T. canis and 19 isolates of T. leonina collected from cats and dogs in different geographical areas of Iran. Mitochondrial genes were amplified by polymerase chain reaction (PCR) and sequenced. Sequence data were aligned using the BioEdit software and compared with published sequences in GenBank. Phylogenetic analysis was performed using Bayesian inference and maximum likelihood methods. Based on pairwise comparison, intra-species genetic diversity within Iranian isolates of T. cati, T. canis and T. leonina amounted to 0-2.3%, 0-1.3% and 0-1.0% for pcox1 and 0-2.0%, 0-1.7% and 0-2.6% for pnad1, respectively. Inter-species sequence variation among the three ascaridoid nematodes was significantly higher, being 9.5-16.6% for pcox1 and 11.9-26.7% for pnad1. Sequence and phylogenetic analysis of the pcox1 and pnad1 genes indicated that there is significant genetic diversity within and among isolates of T. cati, T. canis and T. leonina from different areas of Iran, and these genes can be used for studying genetic variation of ascaridoid nematodes.
本研究旨在确定猫弓首蛔虫、犬弓首蛔虫和狮弓蛔虫分离株内部及相互之间两个线粒体基因，即细胞色素c氧化酶1（pcox1）和NADH脱氢酶1（pnad1）的序列变异。从伊朗不同地理区域的猫和狗身上采集了32株猫弓首蛔虫、9株犬弓首蛔虫和19株狮弓蛔虫，提取其基因组DNA。通过聚合酶链反应（PCR）扩增线粒体基因并进行测序。使用BioEdit软件对序列数据进行比对，并与GenBank中已发表的序列进行比较。采用贝叶斯推断和最大似然法进行系统发育分析。基于成对比较，伊朗猫弓首蛔虫、犬弓首蛔虫和狮弓蛔虫分离株内部pcox1的种内遗传多样性分别为0 - 2.3%、0 - 1.3%和0 - 1.0%，pnad1的种内遗传多样性分别为0 - 2.0%、0 - 1.7%和0 - 2.6%。三种蛔科线虫之间的种间序列变异明显更高，pcox1为9.5 - 16.6%，pnad1为11.9 - 26.7%。pcox1和pnad1基因的序列及系统发育分析表明，来自伊朗不同地区的猫弓首蛔虫、犬弓首蛔虫和狮弓蛔虫分离株内部及相互之间存在显著的遗传多样性，这些基因可用于研究蛔科线虫的遗传变异。
